Anti Adenomatous polyposis coli protein (APC) mAb (Clone 5F2)

CUSABIO

Catalog No.:
CSB-RA951649A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate<br /> All lanes: APC antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 312, 301, 309 kDa<br /> Observed band size: 160 kDa<br />
IHC image of CSB-RA951649A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human, Rat
Clonality Monoclonal (Clone No.: 5F2)

Documents & Links for Anti Adenomatous polyposis coli protein (APC) mAb (Clone 5F2)
Datasheet Anti APC mAb (Clone 5F2) Datasheet

Documents & Links for Anti Adenomatous polyposis coli protein (APC) mAb (Clone 5F2)
Datasheet Anti APC mAb (Clone 5F2) Datasheet
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