Anti ALKBH5 pAb (ATL-HPA007196)

Atlas Antibodies

Catalog No.: ATL-HPA007196-25

Protein Description: AlkB family member 5, RNA demethylase
Gene Name: ALKBH5
Alternative Gene Name: FLJ20308, OFOXD1
Isotype: IgG
Interspecies mouse/rat: ENSMUSG00000042650: 95%, ENSRNOG00000028341: 96%
Entrez Gene ID: 54890
Uniprot ID: Q6P6C2
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Storage Temperature: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Product Specifications

• Application: WB, IHC
• Reactivity: Human
• Clonality: Polyclonal
• Host: Rabbit
• Immunogen: EKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVD
• Gene Sequence: EKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVD
• Gene ID - Mouse: ENSMUSG00000042650
• Gene ID - Rat: ENSRNOG00000028341
• Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.

Citations for Anti ALKBH5 pAb (ATL-HPA007196) – 26 Found

Tirumuru, Nagaraja; Zhao, Boxuan Simen; Lu, Wuxun; Lu, Zhike; He, Chuan; Wu, Li. N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression. Elife. 2016;5( 27371828) 

Zhang, Sicong; Zhao, Boxuan Simen; Zhou, Aidong; Lin, Kangyu; Zheng, Shaoping; Lu, Zhike; Chen, Yaohui; Sulman, Erik P; Xie, Keping; Bögler, Oliver; Majumder, Sadhan; He, Chuan; Huang, Suyun. m(6)A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-like Cells by Sustaining FOXM1 Expression and Cell Proliferation Program. Cancer Cell. 2017;31(4):591-606.e6. 

Chang, Mengqi; Lv, Hongyi; Zhang, Weilong; Ma, Chunhui; He, Xue; Zhao, Shunli; Zhang, Zhi-Wei; Zeng, Yi-Xin; Song, Shuhui; Niu, Yamei; Tong, Wei-Min. Region-specific RNA m(6)A methylation represents a new layer of control in the gene regulatory network in the mouse brain. Open Biology. 2017;7(9) 

Yu, Jun; Chen, Mengxian; Huang, Haijiao; Zhu, Junda; Song, Huixue; Zhu, Jian; Park, Jaewon; Ji, Sheng-Jian. Dynamic m6A modification regulates local translation of mRNA in axons. Nucleic Acids Research. 2018;46(3):1412-1423. 

Wang, Yang; Li, Yue; Yue, Minghui; Wang, Jun; Kumar, Sandeep; Wechsler-Reya, Robert J; Zhang, Zhaolei; Ogawa, Yuya; Kellis, Manolis; Duester, Gregg; Zhao, Jing Crystal. N(6)-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nature Neuroscience. 2018;21(2):195-206. 

Panneerdoss, Subbarayalu; Eedunuri, Vijay K; Yadav, Pooja; Timilsina, Santosh; Rajamanickam, Subapriya; Viswanadhapalli, Suryavathi; Abdelfattah, Nourhan; Onyeagucha, Benjamin C; Cui, Xiadong; Lai, Zhao; Mohammad, Tabrez A; Gupta, Yogesh K; Huang, Tim Hui-Ming; Huang, Yufei; Chen, Yidong; Rao, Manjeet K. Cross-talk among writers, readers, and erasers of m(6)A regulates cancer growth and progression. Science Advances. 2018;4(10):eaar8263. 

Shen, Chao; Sheng, Yue; Zhu, Allen C; Robinson, Sean; Jiang, Xi; Dong, Lei; Chen, Huiying; Su, Rui; Yin, Zhe; Li, Wei; Deng, Xiaolan; Chen, Yinhuai; Hu, Yueh-Chiang; Weng, Hengyou; Huang, Huilin; Prince, Emily; Cogle, Christopher R; Sun, Miao; Zhang, Bin; Chen, Chun-Wei; Marcucci, Guido; He, Chuan; Qian, Zhijian; Chen, Jianjun. RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia. Cell Stem Cell. 2020;27(1):64-80.e9. 

Zhou, Jing; Zhang, Xingli; Hu, Jiajia; Qu, Rihao; Yu, Zhibin; Xu, Hao; Chen, Huifang; Yan, Lichong; Ding, Chenbo; Zou, Qiang; Ye, Youqiong; Wang, Zhengting; Flavell, Richard A; Li, Hua-Bing. m(6)A demethylase ALKBH5 controls CD4(+) T cell pathogenicity and promotes autoimmunity. Science Advances. 2021;7(25) 

Xiao, Dong; Fang, Ting-Xiao; Lei, Ye; Xiao, Sheng-Jun; Xia, Jia-Wei; Lin, Tao-Yan; Li, Yong-Long; Zhai, Jian-Xue; Li, Xiao-Yan; Huang, Shi-Hao; Jia, Jun-Shuang; Tian, Yu-Guang; Lin, Xiao-Lin; Cai, Kai-Can; Sun, Yan. m(6)A demethylase ALKBH5 suppression contributes to esophageal squamous cell carcinoma progression. Aging. 2021;13(17):21497-21512. 

Wang, Anmin; Tao, Wanyiin; Tong, Jiyu; Gao, Juanzi; Wang, Jinghao; Hou, Gaopeng; Qian, Chen; Zhang, Guorong; Li, Runzhi; Wang, Decai; Ren, Xingxing; Zhang, Kaiguang; Ding, Siyuan; Flavell, Richard A; Li, Huabing; Pan, Wen; Zhu, Shu. m6A modifications regulate intestinal immunity and rotavirus infection. Elife. 2022;11( 35098923) 

Feng, Jian; Zhang, Teng; Sorel, Océane; Meng, Wen; Zhang, Xinquan; Lai, Zhao; Yuan, Weiming; Chen, Yidong; Huang, Yufei; Gao, Shou-Jiang. Global profiling reveals common and distinct N6-methyladenosine (m6A) regulation of innate immune responses during bacterial and viral infections. Cell Death & Disease. 2022;13(3):234. 

Hong, Shihao; Shen, Xiaozhong; Cheng, Jinmei; Tang, Hanyu; Sun, Fei. Comprehensive Analysis of the Transcriptome-Wide m6A Methylation in Mouse Pachytene Spermatocytes and Round Spermatids. Frontiers In Genetics. 13( 35368708):832677. 

Hong, Shihao; Shen, Xiaozhong; Luo, Chunhai; Sun, Fei. Comparative analysis of the testes from wild-type and Alkbh5-knockout mice using single-cell RNA sequencing. G3 (Bethesda, Md.). 2022;12(8) 

Tirumuru, Nagaraja; Wu, Li. HIV-1 envelope proteins up-regulate N(6)-methyladenosine levels of cellular RNA independently of viral replication. The Journal Of Biological Chemistry. 2019;294(9):3249-3260. 

Koh, Casslynn W Q; Goh, Yeek Teck; Goh, W S Sho. Atlas of quantitative single-base-resolution N(6)-methyl-adenine methylomes. Nature Communications. 2019;10(1):5636. 

De Jesus, Dario F; Zhang, Zijie; Kahraman, Sevim; Brown, Natalie K; Chen, Mengjie; Hu, Jiang; Gupta, Manoj K; He, Chuan; Kulkarni, Rohit N. m(6)A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes. Nature Metabolism. 2019;1(8):765-774. 

He, Jun-Ju; Li, Zhi; Rong, Zhuo-Xian; Gao, Jie; Mu, Yun; Guan, Yi-Di; Ren, Xin-Xin; Zi, Yu-Yuan; Liu, Li-Yu; Fan, Qi; Zhou, Ming; Duan, Yu-Mei; Zhou, Qin; Deng, Yue-Zhen; Sun, Lun-Quan. m(6)A Reader YTHDC2 Promotes Radiotherapy Resistance of Nasopharyngeal Carcinoma via Activating IGF1R/AKT/S6 Signaling Axis. Frontiers In Oncology. 10( 32850334):1166. 

Wu, Gongwei; Suo, Caixia; Yang, Ying; Shen, Shengqi; Sun, Linchong; Li, Shi-Ting; Zhou, Yingli; Yang, Dongdong; Wang, Yan; Cai, Yongping; Wang, Nana; Zhang, Huafeng; Yang, Yun-Gui; Cao, Jie; Gao, Ping. MYC promotes cancer progression by modulating m(6) A modifications to suppress target gene translation. Embo Reports. 2021;22(3):e51519. 

Chen, Shuliang; Kumar, Sameer; Espada, Constanza E; Tirumuru, Nagaraja; Cahill, Michael P; Hu, Lulu; He, Chuan; Wu, Li. N6-methyladenosine modification of HIV-1 RNA suppresses type-I interferon induction in differentiated monocytic cells and primary macrophages. Plos Pathogens. 2021;17(3):e1009421. 

Tsuchiya, Kazuo; Yoshimura, Katsuhiro; Iwashita, Yuji; Inoue, Yusuke; Ohta, Tsutomu; Watanabe, Hirofumi; Yamada, Hidetaka; Kawase, Akikazu; Tanahashi, Masayuki; Ogawa, Hiroshi; Funai, Kazuhito; Shinmura, Kazuya; Suda, Takafumi; Sugimura, Haruhiko. m(6)A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer. Cancer Gene Therapy. 2022;29(10):1355-1372. 

Liu, Yang; Song, Renjie; Zhao, Lu; Lu, Zhike; Li, Yini; Zhan, Xinyi; Lu, Fengjiao; Yang, Jiang; Niu, Yamei; Cao, Xuetao. m(6)A demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration. Signal Transduction And Targeted Therapy. 2022;7(1):194. 

Wang, Luoluo; Wu, Jian; Liu, Runzhou; Chen, Wenjun; Pang, Zhichang; Zhou, Fan; Xia, Lu; Huang, Jia; Pan, Tao; Su, Xin-Zhuan; Wang, Xiaoyun. Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection. Frontiers In Immunology. 13( 36203583):998756. 

Zhang, Jia-Xiang; Huang, Pei-Jie; Wang, Da-Peng; Yang, Wen-Yu; Lu, Jian; Zhu, Yong; Meng, Xiao-Xiao; Wu, Xin; Lin, Qiu-Hai; Lv, Hui; Xie, Hui; Wang, Rui-Lan. m(6)A modification regulates lung fibroblast-to-myofibroblast transition through modulating KCNH6 mRNA translation. Molecular Therapy : The Journal Of The American Society Of Gene Therapy. 2021;29(12):3436-3448. 

Lee, Ji Hoon; Hong, Juyeong; Zhang, Zhao; de la Peña Avalos, Bárbara; Proietti, Cecilia J; Deamicis, Agustina Roldán; Guzmán G, Pablo; Lam, Hung-Ming; Garcia, Jose; Roudier, Martine P; Sisk, Anthony E; De La Rosa, Richard; Vu, Kevin; Yang, Mei; Liao, Yiji; Scheirer, Jessica; Pechacek, Douglas; Yadav, Pooja; Rao, Manjeet K; Zheng, Siyuan; Johnson-Pais, Teresa L; Leach, Robin J; Elizalde, Patricia V; Dray, Eloïse; Xu, Kexin. Regulation of telomere homeostasis and genomic stability in cancer by N (6)-adenosine methylation (m(6)A). Science Advances. 2021;7(31) 

Phillips, Stacia; Baek, Alice; Kim, Sanggu; Chen, Shuliang; Wu, Li. Protocol for the generation of HIV-1 genomic RNA with altered levels of N (6)-methyladenosine. Star Protocols. 2022;3(3):101616. 

Feng, Jian; Meng, Wen; Chen, Luping; Zhang, Xinquan; Markazi, Ashley; Yuan, Weiming; Huang, Yufei; Gao, Shou-Jiang. N(6)-Methyladenosine and Reader Protein YTHDF2 Enhance the Innate Immune Response by Mediating DUSP1 mRNA Degradation and Activating Mitogen-Activated Protein Kinases during Bacterial and Viral Infections. Mbio. 2023;14(1):e0334922.