Anti AKR1C3 mAb (Clone 4D12)

CUSABIO

Catalog No.:
CSB-RA825204A0HU-50
Immunofluorescence staining of Hela Cells with CSB-RA825204A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing A549 cells stained with CSB-RA825204A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.

Product Specifications
Application IF, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 4D12)

Documents & Links for Anti AKR1C3 mAb (Clone 4D12)
Datasheet Anti AKR1C3 mAb (Clone 4D12) Datasheet

Documents & Links for Anti AKR1C3 mAb (Clone 4D12)
Datasheet Anti AKR1C3 mAb (Clone 4D12) Datasheet
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