Anti ACLY mAb (Clone 3A5)

CUSABIO

Catalog No.:
CSB-RA712206A0HU-50
Western Blot<br /> Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate<br /> All lanes: ACLY antibody at 1:1500<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 121, 120, 92 kDa<br /> Observed band size: 120 kDa<br />
Immunofluorescence staining of HepG2 Cells with CSB-RA712206A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CSB-RA712206A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Product Specifications
Application WB, IF, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 3A5)

Documents & Links for Anti ACLY mAb (Clone 3A5)
Datasheet Anti ACLY mAb (Clone 3A5) Datasheet

Documents & Links for Anti ACLY mAb (Clone 3A5)
Datasheet Anti ACLY mAb (Clone 3A5) Datasheet
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