Anti Acetyl-Histone H3.1 (K14) mAb (Clone 3H3)

CUSABIO

Catalog No.:
CSB-RA010418A14acHU-50
Western Blot<br /> Positive WB detected in Hela whole cell lysate锛孒epG2 whole cell lysate锛孲H-SY5Y whole cell lysate锛孯at brain tissue<br /> All lanes Acetyl-Histone H3.1(K14)antibody at 0.75μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 15 KDa<br /> Observed band size: 15 KDa<br />
Immunocytochemistry analysis of CSB-RA010418A14acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010418A14acHU at 1:46,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product Specifications
Application WB, ICC, IF, ELISA
Reactivity Human, Rat
Clonality Monoclonal (Clone No.: 3H3)

Documents & Links for Anti Acetyl-Histone H3.1 (K14) mAb (Clone 3H3)
Datasheet Anti Acetyl-Histone H3.1 (K14) mAb (Clone 3H3) Datasheet

Documents & Links for Anti Acetyl-Histone H3.1 (K14) mAb (Clone 3H3)
Datasheet Anti Acetyl-Histone H3.1 (K14) mAb (Clone 3H3) Datasheet
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