DPE-1020 - Acrylic Plate

Cosmo Bio

Catalog No.: DCB-306000

This product consists of the "Acrylic Plate" only of DPE-1020

Safety Information

If this device is used or modified in a manner not specified in this manual, then protection afforded by the device will be impaired. Alteration of this device will void the EN61010-1 safety standards warranties and create a potential safety hazard.

Daichi Pure Chemicals is not responsible for any injury or damage caused by use of this device for purposes other than those for which it is intended or by modifications of the device.

The electric operating parameters for this device are as follows:

• Voltage Limit: 0–600 Vdc

• Current Limit: 0–100 mA

• Ambient Temperature Limit: 0–50 °C

Protective gloves and safety glasses must be worn when operating in a laboratory environment. This device is intended for in vitro use only and must be used with its Lid whenever it is operated.

Gel Plates that are separately available (Please refer to Related Products) are made of glass. The Glass-Made Plates are breakable if pressed improperly when attached or detached. Plates should be set again into the device when difficult to be assembled properly.

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Limited Warranty

This device is warranted against manufacturing defects for a period of one year from the original purchase date. DPC will repair/exchange the defective parts under this warranty subject to the terms and conditions of repair of Daichi Pure Chemicals, provided that the device is operated according to the manual and labels.

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I. Features

1. Unique wedge system to firmly and easily fix the Gel Plate

2. Buffer filled in the chambers to cool all the surface of the Gel Plate

3. Smaller quantity of buffer required

4. Easier sample loading visible through the transparent body

5. EN61010-1 safety standards satisfied

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II. Components

Description	Quantity
Lid with power cables	1
Anode Buffer Chamber	1
Cathode Buffer Chamber Frame with electrodes	1
Gasket	2
Wedge	2
Acrylic Plate	2
Instruction manual	1

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III. Specifications

• Code-attached Cover: Acrylic

• Anode Buffer Chamber: Acrylic

• Cathode Buffer Chamber: Acrylic

• Gasket: Silicone

• Wedge: Acrylic

• Acrylic Plate: Acrylic

• Outer Dimensions (L × W × H): 8 × 18 × 18 cm

• Weight (before buffer filled): 0.5 kg

• Precast Gel Compatibility: MULTIGEL-II mini Gels

• Electrical Limits: 600 Vdc, 100 mA

• Buffer Chamber Capacity: 500 mL

Note: This device is not compatible with acetone or ethanol. Use of organic solvents voids the warranties.

Additional equipment and materials required (not included):

• Power supply

• Electrophoresis buffer (Please refer to Related Products)

• Sample loading solution (Please refer to Related Products)

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IV. Unpacking

1. To remove the Lid, hold the device firmly and slide the Lid as indicated on the top.

2. Slide the Wedges towards the center of the Outer Chamber (Anode Buffer Chamber) to release the Inner Chamber (for Acrylic Plates and Cathode Buffer Frame).

3. Take out the Inner Chamber.

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V. Assembly and Operation

Caution: Electrophoresis operates at high voltage and glass-made Gel Plates are fragile by nature; some protective measures like wearing gloves should be taken when handling DPE-1020 not to injure yourself, hands, etc.

1. Ensure that the power supply is turned off and the Lid is not connected.

2. Ensure the Gaskets are set on the Inner Frame (Cathode Buffer Chamber Frame). Otherwise place the Gaskets on the grooves of the Inner Frame and press the Gaskets so that they are fixed firmly to the grooves.

3. Pour the buffer (approximately 200 mL) in the Outer Chamber (Anode Buffer Chamber) up to around 4 cm from the bottom. Then place the Inner Frame into the Outer Chamber, matching respective colors of the plugs (red and black) to those indicated on the Outer Chamber.

4. Fill the Inner Chamber with running buffer up to 2.5 cm below the top edge of the Outer Chamber.
   (Note: Pour the buffer gently to avoid trapping air bubbles along the bottom of the Inner Chamber. Minor bubbles can be ignored.)

5. Load a sample into the well. (Please refer to the Instruction Manual of Gel Plate for preparation of samples.)

6. Ensure the power cables of the Lid are not connected to the power supply.

7. Place the Lid on the Outer Chamber and slide it so that the plugs can be connected with the jacks firmly.

8. Connect the power cables of the Lid to the power supply with the proper polarity.
   (Note: When attaching the Lid, the power supply must be off.)

9. Turn the power supply on to start electrophoresis.

Recommended conditions (for reference):

• SDS-PAGE: 30 mA DC constant for about 60 minutes per Gel Plate

• Native-PAGE: 15 mA DC constant for about 100 minutes per Gel Plate

• DNA-PAGE: 15 mA DC constant for about 100 minutes per Gel Plate

10. Turn the power supply off to stop electrophoresis when the tracking dye (Bromophenol blue or Xylene cyanol FF) reaches approximately 5 mm (around the center of Gasket) from the bottom edge of the Gel Plate(s).

11. Disconnect the power cables of the Lid from the power supply. Holding the device firmly, slide the Lid as indicated on the top for open.

12. Discard the buffers and release the Gel Plate(s) by sliding the Wedges towards the center of the Outer Chamber.

• Staining or other procedures to follow the electrophoresis should be operated according to the individual instructions.

• The device should be washed after each run with a gentle detergent and rinsed thoroughly with distilled or de-ionized water.