4-HNE (4-Hydroxynonenal) ELISA Kit

Reed Biotech

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE . During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE . Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Product Specifications

• Application: ELISA
• Reactivity: All
• Assay Principle: Competitive
• Assay Time: 1.5H
• Measurement Range: 0.63-40 ng/mL
• Sensitivity: 0.38 ng/mL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids