3D Ready Atelocollagen, DMEM, Low Glucose

KOKEN

Catalog No.: KOU-3D-LG01

Background

Stable three-dimensional culture (3D culture) can be performed easily without the need for neutralization or mixing with cell culture medium usually required to form atelocollagen gels. High-purity atelocollagen derived from bovine hide is premixed with DMEM, so it can be used immediately. Simply mix with the cells and warm it up. This product is especially recommended for those beginning the 3D culture without worrying about fibril formation during mixing the solutions or cell sedimentation during gel formation.

Features

• Ready-to-use atelocollagen solution for 3D culture.
• Premixed with DMEM, low glucose or high glucose.
• Easy operation: simply mix with the cells and warm to form a gel.
• Reduces cell sedimentation during gel formation achieves a stable 3D culture.

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.

Citations for 3D Ready Atelocollagen, DMEM, Low Glucose – 7 Found

Sagara, Atsunobu; Miura, Shotaro; Kobinata, Akinori; Naganawa, Risa; Yaginuma, Saki; Saito, Suguru; Saito, Rintaro; Kominato, Hidenori; Yumoto, Tetsuro; Sato, Fumiaki. COL8A1 enhances the invasion/metastasis in MDA-MB-231 cells via the induction of IL1B and MMP1 expression. Biochemical And Biophysical Research Communications. 2023;642( 36577251):145-153. 
- Comments: 3D culture

Iwahashi, Hiroyasu; Kawashima, Yoshihito; Masaki, Hitoshi. Decreased levels of endocytic collagen receptor Endo180 in dermal fibroblasts lead to decreased production of type I collagen and increased expression of matrix metalloproteinase-1. Photodermatology, Photoimmunology & Photomedicine. 2022;38(2):150-157. 
- Comments: 3D culture

Machihara, Kayo; Kageyama, Sou; Oki, Shoma; Makino, Hiroki; Sasaki, Masamichi; Iwahashi, Hiroyasu; Namba, Takushi. Lotus germ extract rejuvenates aging fibroblasts via restoration of disrupted proteostasis by the induction of autophagy. Aging. 2022;14(19):7662-7691. 
- Comments: 3D culture

Fujita, Mayumi; Imadome, Kaori; Somasundaram, Veena; Kawanishi, Miki; Karasawa, Kumiko; Wink, David A. Metabolic characterization of aggressive breast cancer cells exhibiting invasive phenotype: impact of non-cytotoxic doses of 2-DG on diminishing invasiveness. Bmc Cancer. 2020;20(1):929. 
- Comments: 3D culture

Miyazaki, Takuro; Taketomi, Yoshitaka; Higashi, Takayoshi; Ohtaki, Hirokazu; Takaki, Takashi; Ohnishi, Koji; Hosonuma, Masahiro; Kono, Nozomu; Akasu, Risako; Haraguchi, Shogo; Kim-Kaneyama, Joo-Ri; Otsu, Kinya; Arai, Hiroyuki; Murakami, Makoto; Miyazaki, Akira. Hypercholesterolemic Dysregulation of Calpain in Lymphatic Endothelial Cells Interferes With Regulatory T-Cell Stability and Trafficking. Arteriosclerosis, Thrombosis, And Vascular Biology. 2023;43(2):e66-e82. 
- Comments: Miscellaneous

Hanaki, Yusuke; Miyoshi, Sena; Sugiyama, Yasunori; Yanagita, Ryo C; Sato, Masashi. 12-O-Tetradecanoylphorbol 13-acetate promotes proliferation and epithelial-mesenchymal transition in HHUA cells cultured on collagen type I gel: A feasible model to find new therapies for endometrial diseases. Bioscience, Biotechnology, And Biochemistry. 2022;86(10):1417-1422. 
- Comments: Miscellaneous

Yamazaki, Shintaro; Ohka, Fumiharu; Hirano, Masaki; Shiraki, Yukihiro; Motomura, Kazuya; Tanahashi, Kuniaki; Tsujiuchi, Takashi; Motomura, Ayako; Aoki, Kosuke; Shinjo, Keiko; Murofushi, Yoshiteru; Kitano, Yotaro; Maeda, Sachi; Kato, Akira; Shimizu, Hiroyuki; Yamaguchi, Junya; Adilijiang, Alimu; Wakabayashi, Toshihiko; Saito, Ryuta; Enomoto, Atsushi; Kondo, Yutaka; Natsume, Atsushi. Newly established patient-derived organoid model of intracranial meningioma. Neuro-Oncology. 2021;23(11):1936-1948. 
- Comments: Miscellaneous

Documentation Requirements for Order Placement

Import Permit Required for US Customers
This product contains animal-derived, plant-derived, or agricultural materials, such as bovine, porcine, equine, etc., components. These are regulated materials and permits ensure they are imported into the United States in compliance with USDA requirements.
Prior to placing an order, customers are required to apply for a VS 16-3 Import Permit directly from the US government regulatory agency, APHIS (Animal and Plant Health Inspection Service). Please click here for full guidance on applying for an import permit.
Any questions? We are ready to assist you in the process. Please contact us at support@cosmobiousa.com or call us at +1 760 431 4600

Direct Shipment and Fees Authorization
This item must ship directly from our Japan office due to permit requirements, temperature sensitivity, or other shipping considerations. Access the direct shipment authorization form in the Documents section above. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.