PUREfrex® Custom Formulations

PUREfrex® is a reconstituted cell-free protein synthesis system based on purified components derived from E. coli. For researchers with specialized protein expression needs, we offer custom PUREfrex® formulations tailored to your project. Whether you require optimization for difficult-to-express proteins, incorporation of non-standard amino acids, or large-scale reaction formats, our custom formulations can adapt the PUREfrex® platform to fit your unique goals.

This service includes consultation with technical experts to define your expression requirements, custom formulation of reaction components, and testing under controlled conditions to deliver high-yield, high-purity protein. With rapid turnaround and flexible delivery formats, the PUREfrex® custom formulation service supports a wide range of applications including structural biology, functional assays, and synthetic biology.

Let us help you unlock the full potential of cell-free expression with a solution built specifically for your research.

Custom Formulation Example

Diagram illustrating amber suppression using RF1 and E. coli extract.

(-) RF1: For synthesis of a protein containing a noncanonical amino acid by amber suppression as below.
(-) T7 RNA polymerase: For use of other RNA polymerase such as E.coli RNA polymerase.
(-) Specific amino acid(s) and/oraminoacyl-tRNA synthetase: For synthesis of a protein containing noncanonical amino acid or less than 20 amino acids.

Custom Formulation Request Form

PUREfrex Custom Formulation Kit Ordering Information

Agreement:

Custom formulated kits are sold under a usage rights agreement with the Gene Frontier (“GFC”). Usage rights are limited.

Agreement terms (abridged):

  • Materials are for internal research use only, and only for non-clinical use.
  • Material is for non-commercial use. Transfer of materials to any third party is prohibited.
  • Material is not to be modified beyond the Purpose of Use stated in the Agreement.
  • Customer shall promptly notify GFC if any new invention is made through the use of the Material or its components.
  • Any new invention relating to use of the Materials belongs to GFC.
  • Ownership of any products themselves synthesized through the use of the Materials belong to Customer.
  • Industrial use of the Materials for in vitro display purposes is prohibited without a separate “License Agreement.”

Agreement Procedure:

  • Request a custom use agreement by email stating purpose of use from Cosmo Bio USA ([email protected]).
  • Review the Agreement.
  • Request feasibility of your custom design including quantity, packaging, timing, etc.
  • Return the Agreement signed by an authorized signer.
  • Receive a catalog number for your request and pricing from Cosmo Bio USA.
  • Place order using the received catalog number..

Request for Quotation:

  • Prices are set by reaction volume.
  • Sold in 1mL reaction volume increments. Minimum order is 1 mL.
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