Tol1-based Transgenesis Vector
Cosmo Bio
- SKU:
- CSR-CT-NU-002-1
- Shipping:
- Calculated at Checkout
$300.00
Protocol: Recovering plasmid DNA
1. Cut out one of the circles of the paper and immerse it in water or TE in a microfuge tube. Other circles are for backup.
2. Mix by tapping.
3. Centrifuge for 1 minute at >10 krpm.
4. Transform competent bacterial cells (commonly used strains, such as JM109, DH5a and XL1-Blue) with a small amount of supernatant.
5. Spread the bacteria on an LB/agar plate containingl ampicillin, and incubate the plate at 37 oC for >12 hours.
6. Pick up a single colony.
7. Amplify bacteria in liquid media.
8. Extract plsmid DNA by the standard method.
Documents & Links for Tol1-based Transgenesis Vector | |
Datasheet | csr-ct-nu-002-1_tol1-based-transgenesis-vector_datasheet.pdf |
Vendor Page | Tol1-based Transgenesis Vector at Cosmo Bio |
Documents & Links for Tol1-based Transgenesis Vector | |
Datasheet | csr-ct-nu-002-1_tol1-based-transgenesis-vector_datasheet.pdf |
Vendor Page | Tol1-based Transgenesis Vector |
Citations for Tol1-based Transgenesis Vector – 1 Found |
Horstick, Eric J; Jordan, Diana C; Bergeron, Sadie A; Tabor, Kathryn M; Serpe, Mihaela; Feldman, Benjamin; Burgess, Harold A. Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Nucleic Acids Research. 2015;43(7):e48. PubMed |