Gene extraction performance
- Relationship between the amount of each sample (human blood, human PBMC, mouse blood, mouse spleen) added and the expression level of the control gene -
Figure 1. Human blood and mouse blood were 50 mL, and human PBMC and mouse spleen were serially diluted in two-fold increments starting from 5 x 105 cells , and mRNA from leukocytes was extracted/cDNA was synthesized using LeukoComplete™. Housekeeping genes were measured by real-time PCR using the synthesized cDNA as a template. For all sample types, genes were extracted with high linearity (R2 > 0.9) using LeukoComplete™.

Examples of target genes
- Analysis of the expression changes of immune-related genes (IFNG, IL2, IL4, GM-CSF) according to the time of antigen stimulation -
Figure 2. 100 µL of fresh human blood was mixed with CEF peptide pool or PBS, and after incubation at 37°C for 0 to 24 hours, the blood was frozen and stored. After thawing the blood, genes were extracted using LeukoComplete™ and the expression changes of various genes were calculated (each colored line represents the results of a different donor (n=4)). log 2 FC = - (Ct value of target gene [with antigen stimulation] - Ct value of control gene [with antigen stimulation]) - (Ct value of target gene [without antigen stimulation] - Ct value of control gene [without antigen stimulation])