IMMUNO SHOT Immunostaining, Strong

Cosmo Bio

Catalog No.: CSR-IS-S-20

IMMUNO SHOT is an enhancer of antigen-antibody reaction. In Western blotting and ELISA, researchers often experience a weak signal or high background. IMMUNO SHOT improves these problems it as antibody diluents. Due to the principle ofworking mechanism, IMMUNO SHOT can be used in many assay systems that use antigen-antibody reaction.

How IMMUNO SHOT Works

IMMUNO SHOT contains a polymer which, by changing the physicochemical properties of antigen and antibody, enhances the mutual accessibility, and facilitate the specific reaction. The other ingredient, protein, reduces non-specific binding of antibody. Thus, IMMUNO SHOT enhances the antigen-antibody reaction while reduces background

 

Features and Advantages

 1. High signal with low background IMMUNO SHOT enhances the antigen-antibody reaction. Comparing with the method using detergent-containing buffer, several to over 10-fold stronger signal can be obtained while the background level is low. Thus, you can get much higher S/N ratio than usual method.

2. Effective for saving antibody usage and time of reaction time Because higher signal can be obtained using IMMUNO SHOT, you can reduce the amount of antibody used and the time required for reactions.

3. Can be used for many reactions IMMUNO SHOT can be used not only for Western blotting and ELISA, but also forother assay systems using antigen-antibody reactions. In addition, IMMUNO SHOT does not affect activities of HRP (horse radish peroxidase) or AP (alkaline phosphatase), and can be used for assay systems using these enzymes.

4. Easy to use IMMUNO SHOT is formulatedas to Ready to Use. Just exchange your dilution buffer of antibodies to the solutions of IMMUNO SHOT.

 

Citations for IMMUNO SHOT Immunostaining, Strong – 5 Found

Mitsutomi, Shuhei; Sugawara, Anzu; Seki, Masahide; Suzuki, Yutaka; Miyao, Sotaro; Du, Haozhe; Takahashi, Kenji; Mizukami, Yusuke; Taniue, Kenzui; Akimitsu, Nobuyoshi. Nanopore direct RNA sequencing reveals METTL2A-mediated m(3)C sites in poly(A) RNA. Genome Research. 2025;35(11):2406-2417. 

Ozaki, Kazuya; Nagahara, Hiyo; Kawamura, Asaka; Ohgita, Takashi; Higashi, Sachika; Ogura, Kohei; Tsutsuki, Hiroyasu; Iyoda, Sunao; Yokotani, Atsushi; Yamaji, Toshiyuki; Moss, Joel; Yahiro, Kinnosuke. Extracellular Vesicle Inhibitors Enhance Cholix-Induced Cell Death via Regulation of the JNK-Dependent Pathway. Toxins. 2024;16(9) 

Yamaguchi, Yuya; Madhyastha, Harishkumar; Madhyastha, Radha; Choijookhuu, Narantsog; Hishikawa, Yoshitaka; Pengjam, Yutthana; Nakajima, Yuichi; Maruyama, Masugi. Arsenic acid inhibits proliferation of skin fibroblasts, and increases cellular senescence through ROS mediated MST1-FOXO signaling pathway. The Journal Of Toxicological Sciences. 2016;41(1):105-13. 

Ishitani, Shizuka; Inaba, Kenji; Matsumoto, Kunihiro; Ishitani, Tohru. Homodimerization of Nemo-like kinase is essential for activation and nuclear localization. Molecular Biology Of The Cell. 2011;22(2):266-77. 

Queen Intan Nurrahmah et., al.. Nuclear factor-kappa B and JNK mediate macrophage polarization shift induced by C-phycocyanin. Indian Journal of Experimental Biology. 2019;57:381-389.