Human DEFα5 (Defensin Alpha 5, Paneth Cell Specific) ELISA Kit

Reed Biotech

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFα5 . Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human DEFα5 . Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFα5 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 1343 nm. The OD value is proportional to the concentration of Human DEFα5 .You can calculate the concentration of Human DEFα5 in the samples by comparing the OD of the samples to the standard curve.

Product Specifications

• Assay Principle: Sandwich
• Assay Time: 2.5H
• Measurement Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL
• Sample Type: Serum, Plasma, Tissue homogenate and Other biological samples
• Sample Volume: 25 uL