Product Description >/h4>Histostar™ is a two-step IHC staining reagent. This reagent is based on an horseradish peroxidase (HRP) labeled polymer which is conjugated with secondary antibodies. Histostar™ (Rb) is designed to allow Immunohistochemical studies using a user-supplied Rabbit primary antibody. This reagent is available for formalin fixed paraffin-embedded mouse tissue sections.
Immunohistochemistry (IHC) is an efficient means to understand antigens distribution and localization in cells or tissue sections under the microscope. IHC is widely used in clinical diagnosis of disease and basic research purposes. IHC uses monoclonal or polyclonal antibodies to determine the tissue distribution of an antigen of interest. The site of antibody binding is visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold that is directly linked to the primary antibody or to an appropriate secondary antibody. In immunoenzyme technique, frequently used streptavidin-biotin complex (SAB) methods or avidin-biotin complex (ABC) method, which need additional two steps containing both Biotinylated secondary antibody reaction and peroxidase labeled streptavidin reaction after first antibody reaction.
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