EXORPTION(R) Extracellular Vesicles Purification Kit

Cosmo Bio

SKU: SAC-SCI-010

Extracellular vesicles (EVs) are nano-sized particles enclosed by a lipid bilayer membrane secreted from various cells. EVs play important biological roles, including intercellular communication and immune responses. They contain diverse molecular markers such as proteins, nucleic acids, and glycans, both inside and on the surface of the vesicle membrane. These biomarkers strongly reflect the characteristics of their originating cells, making them promising tools for disease diagnosis and therapy.

Ultracentrifuge is the most commonly used method for isolating EVs from biological samples. However, this method requires specialized equipment, is time-consuming, and demands careful operation. Therefore, a simple and efficient EVs isolation method with a shorter processing time is highly desirable.

EXORPTIONTM enables the rapid and high-purity isolation of EVs from biological solutions such as body fluids and conditioned medium. The purified EV can be utilized for various analyses and experiments, including protein and nucleic acid characterization and their biological activities.

Compared to ultracentrifugation, this method has the following advantages:

• Achieved approximately 10 times the amount of EV recovered and high purity
• Simple and takes only about 1/10 the processing time
• Can be purified with approximately 1/100 of the amount of impurities

Kit Contents:

• Purification column: 10 tubes
• LureCAP (attached to the bottom of the Purification column): 10 pieces
• Wash Buffer (×10): 1.7mL (1 tube)
• Elution Buffer: 1.7mL (1 tube)
• Dilution tube: 1 tube
• Product Manual

Additional Required Materials:

• Micropipette
• Pipette tips
• Microcentrifuge tube (2 mL)
• Tabletop centrifuge
• Microcentrifuge
• Vortex mixer
• Low protein absorption grade products are suitable.