The 6×His tag is a synthetic peptide consisting of six consecutive histidine residues (HHHHHH), typically engineered at either the N- or C-terminus of recombinant proteins. This tag enables efficient purification of tagged proteins through immobilized metal affinity chromatography (IMAC), leveraging the strong and selective binding of histidine clusters to metal ions such as Ni²⁺ or Co²⁺.
His-tagged proteins are widely used in recombinant protein production, where the histidine motif’s affinity for Ni²⁺–NTA resins allows purification to near homogeneity in a single chromatographic step. Beyond purification, the His tag facilitates detection, localization, and co-immunoprecipitation of recombinant proteins, particularly when protein-specific antibodies are unavailable.
This monoclonal antibody specifically recognizes the His epitope, providing high sensitivity and specificity in applications such as Western blotting, immunofluorescence, ELISA, and immunoprecipitation. It is suitable for detecting both native and denatured forms of His-tagged proteins, ensuring consistent and reliable results across diverse experimental systems.
