Calcified Nodule Staining kit

Cosmo Bio

Catalog No.: PMC-AK21

Product Description

Calcified Nodule Staining kit Alkaline phosphatase, osteopontin, and osteocalcin are expressedduring the differentiation of bone marrow cells into mature osteoblasts and osteoblasts form calcium tubercle. Alizarin Red S, an anthraquinone derivative, has been usedtoidentify Calcified nodule, because Calcium forms an Alizarin Red S-calcium complex, and the products turn (became) red. Calcified Nodule Staining kit contains Substrate-containing Buffer composed mainly of Alizarin Red S and Chromogenic Substrate,and provides a convenient system for staining calcified nodule with ease.

Citations for Calcified Nodule Staining kit – 5 Found

Kajitani, Naoyo; Yamada, Takaya; Kawakami, Kohei; Matsumoto, Ken-Ichi. TNX deficiency results in bone loss due to an increase in multinucleated osteoclasts. Biochemical And Biophysical Research Communications. 2019;512(4):659-664. 

Fukuda, Naoyuki; Tsuchiya, Akira; Sunarso; Toita, Riki; Tsuru, Kanji; Mori, Yoshihide; Ishikawa, Kunio. Surface plasma treatment and phosphorylation enhance the biological performance of poly(ether ether ketone). Colloids And Surfaces. B, Biointerfaces. 2019;173( 30266018):36-42. 

Miyagi-Shiohira, Chika; Kobayashi, Naoya; Saitoh, Issei; Watanabe, Masami; Noguchi, Yasufumi; Matsushita, Masayuki; Noguchi, Hirofumi. Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells. Cell Medicine. 2017;9(1-2):15-20. 

Wada, Tamaki; Takenawa, Taichi; Komori, Natsuki; Nishimura, Masuhiro; Fujita, Yasutaka; Sawamoto, Osamu. Ascorbic acid enhances the cold preservation period of human adipose tissue-derived mesenchymal stromal cells. Regenerative Therapy. 2023;24( 37868720):154-160. 
- Comments: Cosmo Bio’s Calcified Nodule Staining Kit was used in a study suggesting that supplementation of 3% trehalose- and 5% dextran-40–added lactated Ringer's solution with ascorbic acid enhances the cold preservation of human adipose-derived mesenchymal stem cells, helping to maintain their quality for use in cell therapy.

Takara, Kenyo; Maruo, Naoki; Oka, Kyoko; Kaji, Chiaki; Hatakeyama, Yuji; Sawa, Naruhiko; Kato, Yukinari; Yamashita, Junro; Kojima, Hiroshi; Sawa, Yoshihiko. Morphological study of tooth development in podoplanin-deficient mice. Plos One. 12(2):e0171912. 
- Comments: Cosmo Bio’s Calcified Nodule Staining Kit was used in an electron microscopic study of podoplanin conditional KO mice, revealing no effect on the development and growth of bones and teeth (including on calcification and alkaline phosphatase activity in cultured calvarial osteoblasts), but a possible effect on formation of the osteocyte network created by cell process elongation.