Lymphoprep

Serumwerk Bernburg AG

Catalog No.: AXS-1114544

Density Gradient Medium for the Isolation of Human Mononuclear Cells

Lymphoprep™ density gradent medium offers rapid, simple, and reliable mononucelar cell isolation for basic research using blood samples. Lymphoprep is ready-made, sterile, and endotoxin-tested. Relatively low buoyant density (below 1.077 g/ml) mononuclear cells are retained at the sample/medium interface while more dense erythrocytes and polymorphonuclear leukocytes (PMNs or granulocytes) sediment through the medium.

Lymphoprep density gradent medium is a cost-effective alternative to Ficoll-Paque™ and can be substituted without changing existing protocols.

About Lymphoprep

A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Bøyum in 1968. For more than 35 years Lymphoprep medium has been widely used for isolating mononuclear cells (monocytes and lymphocytes) by exploiting their low buoyant density (below 1.077 g/ml) as compared to erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). Mononuclear cells are retained at the sample/medium interface while erythrocytes and PMNs sediment through the medium.

The described method is rapid, simple and reliable and gives excellent results with blood samples from normal individuals and patients. To obtain maximum yields it is important that blood samples are diluted 1:1 with physiological saline before being applied to Lymphoprep. Erythrocyte contamination in mononuclear cell suspensions is usually between 3-10% of the total cell number. Some immature PMNs may sediment with lymphocytes during intense immunosuppressive therapy. Removal of most platelets from mononuclear cell preparations is essential to avoid inhibition in cytotoxicity tests. Lymphoprep™ can be used for the preparation of pure lymphocyte suspensions for tissue typing, anti-lymphocyte sera and immunological research. Thorsby and Brattelie used this technique with only slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.

Each batch of Lymphoprep is assessed for endotoxin level using a specific LAL test. The manufacturer's goal is to produce batches with an endotoxin level lower or equal to 0.13 EU/ml.

Specifications

• Sodium diatrizoate      9.1% (w/v)
• Polysaccharide            5.7% (w/v)
• Density                        1.077 ± 0.001 g/mL
• Osmolality                  290 ± 15 mOsm
• Endotoxin                   <1.0 EU/mL
• Sterility                       claimed according to Ph.Eur

Lymphoprep is manufactured, packaged and released in compliance with:

• Current EU Guide to Good Manufacturing Practice
• Fresenius Kabi AS Quality System
• Fresenius Kabi AS Manufacturing License

For every lot, a Certificate of Analysis showing the actual values for density, osmolality and endotoxin level. Please contact us for the Certificate of Analysis.