Anti-Parvalbumin pAb is prepared from rabbits immunized with purified rat skeletal muscle parvalbumin.
Validated for western blot, immunoprecipitation, ELISA, immunocytochemistry, immunofluorescence and IHC(p), this antibody is useful for immunohistochemical and biochemical studies of parvalbumin in diverse muscular and neurodegenerative diseases.
Background
Neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease have been increasing rapidly and have become a serious social problem. In recent years, new causative genes have been discovered for amyotrophic lateral sclerosis (ALS) and other intractable neurological diseases opening new avenues for research on pathogenesis. It has been suggested that aggregation and accumulation of specific proteins cause neurotoxicity and the formation of lesions, but the onset and progression mechanisms are still unclear. Neuropathological diagnostic and experimental model biomarkers are needed for drug construction, drug discovery, and therapeutic development.
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurons which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.
Antigen/Source
Purified parvalbumin from rat skeletalmuscle.
Host
Rabbit
Immunogen
Rat
Reacts with
Human/Rat
Isotype
IgG
Fraction
Affinity Purified
Applications
Western Blot/IHC paraffin embedding section/Enzyme Linked Immunosorbent Assay/Immuno Fluorescence/Immunocytochemistry (cell)/Immunoprecipitation
Shape
Liquid
Preservative
0.1% NaN3
Storage
-20C
References
Inaguma Y, Kurobe N, Shinohara H, Kato K. (1991) Sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes. Biochim Biophys Acta. 1075: 68-74.
Product Specifications
Application
ELISA, ICC, IP, IHC(p), WB, IF
Reactivity
Human, Rat
Clonality
Polyclonal
Host
Rabbit
Documents & Links for Anti Parvalbumin Alpha pAb (Rabbit, Affinity Purified)
