Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)

Catalog No:
SGE-CFT45325
$654.00

This immuno-staining cocktail of three differentially labeled collagen isotype-specific rat monoclonal antibodies helps in analyses of renal and skin biopsies to distinguish Alport syndrome from unaffected individuals.

Two clones (H53, B51) labeled with FITC reveal the diagnostic collagen α5(IV) while a third (H25), labeled with Texas Red, detects collagen α2(IV), revealing basement membrane structure.

Click here to browse a well organized list of products for Bone, Collagen, and Extracellular Matrix research.

Check out our Human Collagen IVα Rat Monoclonal Antibodies Dashboard for more information about this product and additional epitope-defined monoclonal antibodies useful for research on hereditary diseases related to collagen IV.


Alport syndrome is an inherited disease characterized by the pathological absence or reduction of the collagen α5(IV) chain in glomerular basement membrane (GBM), tubular basement membrane (TBM) and Bowman's capsular basement membrane. Anti-Collagen 4 Cocktail mAb (cat no. SGE-CFT45325) is a cocktail of three different rat mAbs for easy and rapid staining of human renal and skin biopsy sections to distinguish Alport syndrome from normal tissue. It comprises two different FITC-conjugated mAbs (clones H53 and B51) that reveal the Alport-affected α5(IV) chain in glomerular, tubular and Bowman's capsular BMs and an internal positive control Texas Red-conjugated mAb (clone H25) that targets α2(IV) to reveal endothelial basement membrane structure


Product specifications

Specification Detail
Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)
Cat No SGE-CFT45325
Application Staining of human cryostat sections by direct immunofluorescence (acid-urea treatment not required)
Quantity 1.0 ml
Appearance Solution, 0.1% NaN3 as preservative
Purification Affinity chromatography
Fluorescent labeling H53 and B51, FITC; H25, Texas Red
Clone names H53 (rat IgG2a/kappa), B51 (rat IgG2a) and H25 (rat IgG1/kappa)
Specificity H53 is specific to imperfection III of α5(IV) (Reference 1); B51 is specific to NC1 domain of α5(IV) (Reference 2); H25 is specific to imperfection XIII of α2(IV) (Reference 1).
Antibodies preparation Monoclonal antibodies were raised in rats against synthetic peptides and the native NC1 domain of type IV collagen.
Storage In the dark at 2-4°C, or below -30°C. Stable at these conditions for several years
Source Shigei Medical Research Institute, 2117 Yamada, Okayama 701-0202, Japan; TEL: +81-86-282-3113; FAX: +81-86-282-3115; E-mail: inst@shigei.or.jp

References

  1. Kagawa M et al.
    Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport syndrome.
    Nephrol. Dial. Transplant. 12: 1238-1241 (1997) (PMID: 9198058)
  2. Borza DB et al.
    The NCI domain of collagen IV encodes a novel network composed of the α1, α2, α5, and α6 chains in smooth muscle basement membranes.
    J. Biol. Chem. 276: 28532-28540 (2001) (PMID: 11375996)
  3. Sado et al.
    Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different α chains of human type IV collagen.
    Histochem. Cell Biol. 104: 267-275 (1995) (PMID: 8548560)
  4. Yoshioka K et al.
    Type IV collagen α5 chain: Normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody.
    Am. J. Pathol. 144: 986-996 (1994) (PMID: 8178947)
  5. Ninomiya Y et al.
    Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies.
    J. Cell Biol. 130: 1219-1229 (1995) (PMID: 7657706)
  6. Naito I et al.
    Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome.
    Kidney Int. 50: 304-311 (1996) (PMID: 8807602)

Citations

  1. Bu L et al.
    Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome.
    Kidney Int Rep. 14(4): 1031-1035 (2019) (PMID: 31312776)
  2. Samar M et al.
    Negative Staining for COL4A5 Correlates With Worse Prognosis and More Severe Ultrastructural Alterations in Males With Alport Syndrome.
    Kidney Int Rep. 2(1): 44-52 (2017) (PMID: 29142939)
  3. Malone AF et al.
    Functional assessment of a novel COL4A5 splice region variant and immunostaining of plucked hair follicles as an alternative method of diagnosis in X-linked Alport syndrome.
    Pediatr Nephrol. 32(6): 997-1003 (2017) (PMID: 28013382)
  4. Nozu K et al.
    X-linked Alport syndrome caused by splicing mutations in COL4A5.
    Am Soc Nephrol. 9(11): 1958-64 (2014) (PMID: 25183659)
  5. Matsubara S et al.
    Pregnancy complicated with Alport syndrome: a good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining.
    Obstet Gynaecol Res. 35(6): 1109-14 (2009) (PMID: 20144175)
  6. Patey-Mariaud de Serre N et al.
    Collagen alpha5 and alpha2(IV) chain coexpression: analysis of skin biopsies of Alport patients.
    Kidney Int. 72(4): 512-6 (2007) (PMID: 17554254)
  7. Kharrat M et al.
    Autosomal dominant Alport's syndrome: study of a large Tunisian family.
    Kidney Dis Transpl. 17(3): 320-5 (2006) (PMID: 16970251)
Documents & Links for Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)
Datasheet sge-cft45325_anti-collagen-4-cocktail-mab-clones-h53-b51-h25_datasheet.pdf
Datasheet Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) Datasheet

Documents & Links for Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25)
Datasheet sge-cft45325_anti-collagen-4-cocktail-mab-clones-h53-b51-h25_datasheet.pdf
Datasheet Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) Datasheet

Citations for Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) – 1 Found
Bu et al. 2019. Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome. KI Reports. :.  Journal