For nerve research on nerve regeneration and demyelinating diseases!

Schwann Cell Line

Mouse Schwann cell line IMS32 is an immortalized cell line established from the dorsal root ganglion and peripheral nerve tissue of mature ICR mice. It has many biochemical characteristics. Please use it for nerve research such as nerve regeneration and demyelinating diseases.

Rat Schwann cell line IFRS1 expresses glial cell proteins such as S100 and GFAP. , myelination ability 1), 2), 3) have been confirmed. There is no other example of myelination using established cell lines, so please use it as a useful tool to analyze the functions of Schwann cells in neurodegenerative diseases and axonal regeneration.

 

Background

Schwann cells are cells that form and maintain the myelin sheath that surrounds the axons of peripheral nerves, and also produce cytokines that are important for the maintenance of motor and sensory neurons. In recent years, it has become clear that Schwann cells play a major role in axonal guidance and repair in the process of axonal regeneration after nerve injury. Abnormalities in Schwann cells have also attracted attention as a causative factor of amyotrophic lateral sclerosis (ALS)

Product data

Anti-p75 antibody fluorescent staining (green: p75, blue: nuclear staining)Anti-S100 antibody fluorescent staining (green: S100, blue: nuclear staining)Phase-contrast microscope image
(click image to enlarge)

Figure 1. Fluorescent immunostaining and phase-contrast microscope image
Left) Anti-p75 antibody fluorescent staining (green: p75, blue: nuclear staining)
Center) Anti-S100 antibody fluorescent staining (green: S100, blue: nuclear staining)
Right) Phase-contrast microscope image
Magnification: X100

References
  1. Sango K, Yanagisawa H, Kawakami E, et al. Spontaneously immortalized Schwann cells from adult Fischer rat as a valuable tool for exploring neuron-Schwann cell interactions. J Neurosci Res 2011;89:898-908.
  2. Sango K, Kawakami E, Yanagisawa H, et al. Myelination in coculture of established neuronal and Schwann cell lines. Histochem Cell Biol 2012;137:829-839.
  3. Sango, Kazunori, et al. "Immortalized adult rodent Schwann cells as in vitro models to study diabetic neuropathy." Experimental diabetes research 2011
Catalog Number Product Name Size
PMC-SWN-IFRS1C Schwann Cell Line (IFRS1), rat 1 Vial
PMC-SWN-IMS32C Schwann Cell Line (IMS32), mouse 1 Vial

Please Note

  • Regarding this product, based on the license agreement with the Tokyo Metropolitan Institute of Medical Science, use it only for your own research purpose, prohibiting the provision of cells to third parties (distribution, lending, transfer, permission to use, etc.) To do.
  • Cells are confirmed mycoplasma negative.
  • If the frozen cells will not be used immediately after receipt, store them in liquid nitrogen.
  • Please use cells together with each dedicated medium.
Catalog Number Product Name Size
PMC-SWN-MM Schwann Cell (IMS32) Medium 500 ml
PMC-SWNMR Schwann Cell (IFRS1) Medium 250 ml