Cell / Cell culture products
VAC Visceral Adipocytes (rats)
Stromal-Vascular fraction from rat mesenteric adipose tissue VAC Visceral adipocytes (rat)
VAC (Visceral Adipocyte) is a product that enables the cultivation of differentiation-inducing system of visceral adipocytes, which was originally developed as an epoch-making tool indispensable for the development of lifestyle-related disease therapeutic drugs and preventive foods.
Background
Excessive accumulation of visceral fat is the main factor in the development of lifestyle-related diseases such as diabetes. VAC (Visceral Adipocyte) has made it possible to elucidate the mechanism of visceral fat accumulation from the visceral adipocyte culture level.
Although the development of functional foods and therapeutic drugs that suppress the excessive accumulation of visceral fat has been eagerly desired, no differentiation induction system for "visceral adipocytes" in a culture system has been developed so far. Therefore, there were many unclear points, such as what mechanism causes visceral fat accumulation, which cells form visceral adipocytes, and what processes lead to early differentiation and induction of differentiation. .
The VAC produced by our cell culture technology has established a visceral adipocyte differentiation induction system, making it possible to elucidate the mechanism of visceral fat accumulation that causes lifestyle-related diseases from the visceral adipocyte culture level.
Please use it for the search for therapeutic drugs for obesity, diabetes, hypertension, and arteriosclerosis, the development of foods to prevent lifestyle-related diseases, and the functional testing of anti-obesity functional foods.
It is a cell group containing mesenteric preadipocyte cells and vascular endothelial cells collected from rat mesentery, and a culture solution product optimized for mesenteric adipocyte differentiation developed by our company. It can be used for research on all processes from preadipocytes to mature adipocytes.
Intended use
- Exploring therapeutic agents for obesity, diabetes, hypertension, and arteriosclerosis
- Development of preventive food for lifestyle-related diseases, functional test of anti-obesity functional food
Features
- Differentiation inducers such as indomethacin, dexamethasone, and PPAR-γ agonists were not added.
- Natural lipids are added as visceral adipocyte differentiation inducers.
- We use serum from which visceral adipocyte differentiation inhibitory factor (VAI) activity has been removed.
- More than 80% of the cells differentiate into visceral adipocytes.
Composition
| Organization | Animal | Age in weeks | Composition | Catalog number |
| mesenteric adipose tissue | SD rat | mature animal | Visceral adipose precursor cells (frozen cells) 3×10 6 cells 1 cell | PMC-VAC01C |
| frozen cells | ||||
| Visceral adipose precursor cells (frozen cells) 1.5×10 6 cells 1 | PMC-VACH1C | |||
| product number: half size of VAC01C | frozen cells |
Frozen Cells : The cells are delivered frozen in dry ice packaging. After you receive the product, you can start culturing immediately, or can freeze it at the temperature specified in the instruction manual.
Example of usage/Product Data
Figure 1 Development process of visceral adipocytes (when cultured from frozen cells)
Figure 2 Changes in adiponectin secretion
Experimental example: Browning of white (visceral) adipocytes
We investigated the browning of white fat using visceral fat cells (product number VAC01C).
As a result of adding noradrenaline to visceral adipocytes, the UCP-1 gene increased rapidly within 6 hours, and UCP-1 protein expression was also confirmed. This showed browning of white adipocytes in the culture system, suggesting that visceral adipocytes have a thermogenic mechanism.
For details, please view here (Presentation at the symposium "New Developments in Brown Fat Research").
VAC Visceral fat cells (frozen cells)
| Product name | Catalog number | Size |
| Rat visceral adipocyte V-1 / Visceral Adipocyte V-1 , Rat |
PMC-VAC01C | 1 VIAL [3 x 106 cells x 1] |
| Rat visceral adipocyte H-1/ Visceral Adipocyte H-1 , Rat | PMCVACH1C |
1 VIAL |
- If the frozen cells will not be used immediately after receipt, store them in liquid nitrogen (or below -70°C).
- Please use the cells together with the dedicated medium.
Dedicated medium
A serum-containing medium whose components have been adjusted so that preadipocytes can be induced to differentiate into adipocytes.
Visceral adipose differentiation medium ver . See here
for a detailed explanation of Visceral Fat Differentiation Medium ver. 2 .
| Ingredient content | Visceral fat differentiation medium Ver. 1 |
Visceral fat differentiation medium Ver. 2 |
| forced differentiation agent | Additive-free | Additive-free |
| fatty acid | addition | addition |
| serum | addition | addition |
| insulin concentration | 10,000 ng/mL | 1 ng/mL |
| Other growth hormones | Additive-free | Addition (physiological concentration) |
| Product name | Catalog number | Size |
| Visceral Adipocyte Culture Medium Ver.1 | PMC-VACMR | 250ML |
| Visceral Adipocyte Culture Medium Ver.2 | PMC-VACM2 | 250ML |
Related products
- Visceral fat differentiation medium Cosmedium
- GPDH activity assay kit
- Lipit Assay Kit (Oil Red O staining)
- Adipocyte fluorescent staining kit
- Real-time PCR primer set (for adipocytes/tissues)
- POLARIC™ Live Cell Staining Fluorescent Dye
- DNA quantification kit
- collagen quantification kit
- Type I Collagenase Assay Kit
- 2-deoxyglucose (2DG) metabolic rate measurement kit
- Gelatin Zymo/Fluorescent Labeled Gelatin Zymo Electrophoresis Kit