Anti CD44 Antigen v9 mAb (Clone RV3)

Cosmo Bio

Catalog No.: CAC-LKG-M003

Background

CD44 is a single-pass type I transmembrane protein and functions as a cellular adhesion molecule for hyaluronic acid, a major component of the extracellular matrix. It exists in numerous isoforms that are generated through alternative splicing of CD44 precursor mRNA. Whereas the standard isoform of CD44 (CD44s) is expressed predominantly in hematopoietic cells and normal epithelial cell subsets, CD44v (variant) isoforms, which contain additional insertions in the membrane-proximal extracellular region, are highly expressed in epithelial-type carcinomas. Moreover, CD44 is reported to be a cell surface marker for cancer stem cells (CSCs) derived from solid tumors including breast, prostate, colon, head and neck and pancreatic cancer. Expression of CD44, especially variant isoforms (CD44 v8-10), contributes to reactive oxygen species (ROS) defense through upregulation of the synthesis of reduced glutathione (GSH), the primary intracellular antioxidant. CD44 v8-10 interacts with and stabilizes xCT, a subunit of the cystine-glutamate transporter xc(-), and thereby promotes cystine uptake for GSH synthesis. The ability to avoid the consequences of exposure to high levels of ROS is required for cancer cell survival and propagation in vivo. CSCs (whose defense against ROS is enhanced by CD44v8-10) are thus thought to drive tumor growth, chemoresistance and metastasis. Clone RV3 (a monoclonal antibody specific for human CD44 v9) can be used in flow cytometry, and importantly, for the enrichment of CSCs using FACS. RV3 can be applied towards understanding a variety of molecular mechanisms and towards the development of new medicines against cancer stem cells using in vitro cell-based assays such as the "in vitro sphere formation" and "in vivo lung metastasis" assays.

References

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Product Specifications

• Application: FC, IP, ELISA, IF, IHC(p), WB, ICC
• Reactivity: Human
• Clonality: Monoclonal (Clone No.: RV3)
• Host: Rat
• Source: Monoclonal
• Purification: Ig-PG

Citations for Anti CD44 Antigen v9 mAb (Clone RV3) – 23 Found

Jeong, Sangho; Choi, Eunyoung; Petersen, Christine P; Roland, Joseph T; Federico, Alessandro; Ippolito, Rossana; D'Armiento, Francesco P; Nardone, Gerardo; Nagano, Osamu; Saya, Hideyuki; Romano, Marco; Goldenring, James R. Distinct metaplastic and inflammatory phenotypes in autoimmune and adenocarcinoma-associated chronic atrophic gastritis. United European Gastroenterology Journal. 2017;5(1):37-44. 

Ogihara, Koichiro; Kikuchi, Eiji; Okazaki, Shogo; Hagiwara, Masayuki; Takeda, Toshikazu; Matsumoto, Kazuhiro; Kosaka, Takeo; Mikami, Shuji; Saya, Hideyuki; Oya, Mototsugu. Sulfasalazine could modulate the CD44v9-xCT system and enhance cisplatin-induced cytotoxic effects in metastatic bladder cancer. Cancer Science. 2019;110(4):1431-1441. 

Takeda, Mitsunobu; Koseki, Jun; Takahashi, Hidekazu; Miyoshi, Norikatsu; Nishida, Naohiro; Nishimura, Junichi; Hata, Taishi; Matsuda, Chu; Mizushima, Tsunekazu; Yamamoto, Hirofumi; Ishii, Hideshi; Doki, Yuichiro; Mori, Masaki; Haraguchi, Naotsugu. Disruption of Endolysosomal RAB5/7 Efficiently Eliminates Colorectal Cancer Stem Cells. Cancer Research. 2019;79(7):1426-1437. 

Osaki, Luciana H; Bockerstett, Kevin A; Wong, Chun F; Ford, Eric L; Madison, Blair B; DiPaolo, Richard J; Mills, Jason C. Interferon-γ directly induces gastric epithelial cell death and is required for progression to metaplasia. The Journal Of Pathology. 2019;247(4):513-523. 

Tokunaga, Eriko; Fujita, Aya; Takizawa, Katsumi; Baba, Kimiko; Akiyoshi, Sayuri; Nakamura, Yoshiaki; Ijichi, Hideki; Masuda, Takanobu; Koga, Chinami; Tajiri, Wakako; Ohno, Shinji; Taguchi, Kenichi; Ishida, Mayumi. CD44v9 as a poor prognostic factor of triple-negative breast cancer treated with neoadjuvant chemotherapy. Breast Cancer (Tokyo, Japan). 2019;26(1):47-57. 

Aguilar-Chaparro, Mario Alejandro; Rivera-Pineda, Sonia Andrea; Hernández-Galdámez, Hury Viridiana; Piña-Vázquez, Carolina; Villa-Treviño, Saúl. The CD44std and CD44v9 subpopulations in non-tumorigenic invasive SNU-423 cells present different features of cancer stem cells. Stem Cell Research. 2023;72( 37844417):103222. 

Fontanella, Rosaria A; Sideri, Silvia; Di Stefano, Chiara; Catizone, Angiolina; Di Agostino, Silvia; Angelini, Daniela F; Guerrera, Gisella; Battistini, Luca; Battafarano, Giulia; Del Fattore, Andrea; Campese, Antonio Francesco; Padula, Fabrizio; De Cesaris, Paola; Filippini, Antonio; Riccioli, Anna. CD44v8-10 is a marker for malignant traits and a potential driver of bone metastasis in a subpopulation of prostate cancer cells. Cancer Biology & Medicine. 2021;18(3):788-807. 

Jogo, Tomoko; Oki, Eiji; Nakanishi, Ryota; Ando, Koji; Nakashima, Yuichiro; Kimura, Yasue; Saeki, Hiroshi; Oda, Yoshinao; Maehara, Yoshihiko; Mori, Masaki. Expression of CD44 variant 9 induces chemoresistance of gastric cancer by controlling intracellular reactive oxygen spices accumulation. Gastric Cancer : Official Journal Of The International Gastric Cancer Association And The Japanese Gastric Cancer Association. 2021;24(5):1089-1099. 

Thanee, Malinee; Padthaisong, Sureerat; Suksawat, Manida; Dokduang, Hasaya; Phetcharaburanin, Jutarop; Klanrit, Poramate; Titapun, Attapol; Namwat, Nisana; Wangwiwatsin, Arporn; Sa-Ngiamwibool, Prakasit; Khuntikeo, Narong; Saya, Hideyuki; Loilome, Watcharin. Sulfasalazine modifies metabolic profiles and enhances cisplatin chemosensitivity on cholangiocarcinoma cells in in vitro and in vivo models. Cancer & Metabolism. 2021;9(1):11. 

Fujimoto, Takuya; Tsunedomi, Ryouichi; Matsukuma, Satoshi; Yoshimura, Kiyoshi; Oga, Atsunori; Fujiwara, Nobuyuki; Fujiwara, Yasuhiro; Matsui, Hiroto; Shindo, Yoshitaro; Tokumitsu, Yukio; Suzuki, Nobuaki; Kobayashi, Shogo; Hazama, Shoichi; Eguchi, Hidetoshi; Nagano, Hiroaki. Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes. Oncology Letters. 2021;21(1):30. 

Ueda, Shinobu; Takanashi, Masakatsu; Sudo, Katsuko; Kanekura, Kohsuke; Kuroda, Masahiko. miR-27a ameliorates chemoresistance of breast cancer cells by disruption of reactive oxygen species homeostasis and impairment of autophagy. Laboratory Investigation; A Journal Of Technical Methods And Pathology. 2020;100(6):863-873. 

Goto, Nohara; Suzuki, Hiroyuki; Zheng, Ling; Okano, Yasuhito; Okita, Yukari; Watanabe, Yukihide; Kato, Yukinari; Kato, Mitsuyasu. Promotion of squamous cell carcinoma tumorigenesis by oncogene-mediated THG-1/TSC22D4 phosphorylation. Cancer Science. 2023;114(10):3972-3983. 

Takizawa, Kohei; Muramatsu, Koji; Maruyama, Kouji; Urakami, Kenichi; Sugino, Takashi; Kusuhara, Masatoshi; Yamaguchi, Ken; Ono, Hiroyuki; Kitagawa, Yuko. Metabolic Profiling of Human Gastric Cancer Cells Treated With Salazosulfapyridine. Technology In Cancer Research & Treatment. 2020;19:1533033820928621. 

Trinh, Vincent Quoc-Huy; Ankenbauer, Katherine E; Liu, Jiayue; Batardiere, Maelle; Maurer, H Carlo; Copeland, Celina; Wong, Jahg; Ben-Levy, Olivia; Torbit, Sabrina M; Jarvis, Brenda; Revetta, Frank; Ivanov, Sergey; Jyotsana, Nidhi; Makino, Yuki; Ruelas, Amanda M; Means, Anna L; Maitra, Anirban; Tan, Marcus C B; DelGiorno, Kathleen E. Oncogenic GNAS drives a gastric pylorus program in intraductal papillary mucinous neoplasms of the pancreas. Biorxiv : The Preprint Server For Biology. 2024; 38464029 

Druffner, Sara R; Venkateshwaraprabu, Shrinidhi; Khadka, Stuti; Duncan, Benjamin C; Morris, Maeve T; Sen-Kilic, Emel; Damron, Fredrick H; Liechti, George W; Busada, Jonathan T. Comparison of gastric inflammation and metaplasia induced by Helicobacter pylori or Helicobacter felis colonization in mice. Microbiology Spectrum. 2024;12(6):e0001524. 

Monteleone, Lorenzo; Speciale, Andrea; Valenti, Giulia Elda; Traverso, Nicola; Ravera, Silvia; Garbarino, Ombretta; Leardi, Riccardo; Farinini, Emanuele; Roveri, Antonella; Ursini, Fulvio; Cantoni, Claudia; Pronzato, Maria Adelaide; Marinari, Umberto Maria; Marengo, Barbara; Domenicotti, Cinzia. PKCα Inhibition as a Strategy to Sensitize Neuroblastoma Stem Cells to Etoposide by Stimulating Ferroptosis. Antioxidants (Basel, Switzerland). 2021;10(5) 

Jeong, Haengdueng; Lee, Buhyun; Kim, Kwang H; Cho, Soo Young; Cho, Yejin; Park, Jeongeun; Lee, Yura; Oh, Yeseul; Hwang, Bo Ram; Jang, Ah-Ra; Park, Jong-Hwan; Park, Ji-Ho; Jeong, Sang-Ho; Lee, Daekee; Lee, Yong Chan; Lim, Kyung-Min; Goldenring, James R; Nam, Ki Taek. WFDC2 Promotes Spasmolytic Polypeptide-Expressing Metaplasia Through the Up-Regulation of IL33 in Response to Injury. Gastroenterology. 2021;161(3):953-967.e15. 

Krishnan, Vaidehi; Lim, Debbie Xiu En; Hoang, Phuong Mai; Srivastava, Supriya; Matsuo, Junichi; Huang, Kie Kyon; Zhu, Feng; Ho, Khek Yu; So, Jimmy Bok Yan; Khor, Christopher; Tsao, Stephen; Teh, Ming; Fock, Kwong Ming; Ang, Tiing Leong; Jeyasekharan, Anand D; Tan, Patrick; Yeoh, Khay-Guan; Ito, Yoshiaki. DNA damage signalling as an anti-cancer barrier in gastric intestinal metaplasia. Gut. 2020;69(10):1738-1749. 

Ohtsuka, Junko; Oshima, Hiroko; Ezawa, Issei; Abe, Ryo; Oshima, Masanobu; Ohki, Rieko. Functional loss of p53 cooperates with the in vivo microenvironment to promote malignant progression of gastric cancers. Scientific Reports. 2018;8(1):2291. 

Chano, Tokuhiro; Kita, Hiroko; Avnet, Sofia; Lemma, Silvia; Baldini, Nicola. Prominent role of RAB39A-RXRB axis in cancer development and stemness. Oncotarget. 2018;9(11):9852-9866. 

Nishino, Makoto; Ozaki, Mari; Hegab, Ahmed E; Hamamoto, Junko; Kagawa, Shizuko; Arai, Daisuke; Yasuda, Hiroyuki; Naoki, Katsuhiko; Soejima, Kenzo; Saya, Hideyuki; Betsuyaku, Tomoko. Variant CD44 expression is enriching for a cell population with cancer stem cell-like characteristics in human lung adenocarcinoma. Journal Of Cancer. 8(10):1774-1785. 

Choi, Eun-Seok; Kim, Hyunjin; Kim, Hyung-Pyo; Choi, Yongdoo; Goh, Sung-Ho. CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer. Scientific Reports. 2017;7(1):4930. 

Quoc-Huy Trinh, Vincent; Ankenbauer, Katherine E; Torbit, Sabrina M; Taranto, Christopher P; Liu, Jiayue; Batardiere, Maelle; Kumar, Bhoj; Maurer, H Carlo; Revetta, Frank; Chen, Zhengyi; Kruse, Angela R S; Judd, Audra M; Copeland, Celina; Wong, Jahg; Ben-Levy, Olivia; Jarvis, Brenda; Brown, Monica; Brown, Jeffrey W; Das, Koushik; Makino, Yuki; Spraggins, Jeffrey M; Lau, Ken S; Azadi, Parastoo; Maitra, Anirban; Tan, Marcus C B; DelGiorno, Kathleen E. Mutant GNAS drives a pyloric metaplasia with tumor suppressive glycans in intraductal papillary mucinous neoplasia. Cell Reports. 2025;44(12):116684.