"Linking Ubiquitin Research to Drug Discovery"

Ubiquigent - Ubiquitin Binding Proteins

Ubiquitin chains may be formed through the linkage of the C-termini of one (distal) ubiquitin to one of seven (K6, K11, K27, K29, K33, K48 or K63) Lysyl side chains or to the alpha-amino group on the N-terminus of a proximal ubiquitin; and so on to generate a chain. The specificity of ubiquitin signaling is then achieved or ’decoded’ by the interaction of mono-ubiquitylated or poly-ubiquitylated target proteins with specific ubiquitin-chain binding proteins (of which around 200 are thought to exist) resulting in various specific biochemical cascades.

Thus ubiquitin chain binding proteins may be used to capture substrate proteins linked to ubiquitin chains of specific linkage(s). A summary of the characteristics and applications of selected ubiquitin binding proteins offered by Ubiquigent are summarized below:

NEMO and Optineurin: Capture both K63 and linear polyubiquitin chains (free or linked to substrate proteins). A single mutation in these proteins (D311N for NEMO and D474N for Optineurin) abolishes their polyubiquitin chain binding activity and thus these variants may be used as controls in capture experiments.

S5a: Captures K48, K63 and K11 poly-ubiquitin chains.

Catalog Number	Product Name	Size	Datasheet
UBI-66-1002-050	NEMO [GST-tagged]	50 ug	Datasheet
UBI-66-1005-050	Optineurin [GST-tagged]	50 ug	Datasheet
UBI-66-1013-050	NEMO (D311N) [GST-tagged]	50 ug	Datasheet
UBI-66-1014-050	Optineurin (D474N) [GST-tagged]	50 ug	Datasheet
UBI-66-1015-050	TOM1 [GST-tagged]	50 ug	Datasheet
UBI-66-1016-050	TOLLIP [GST-tagged]	50 ug	Datasheet
UBI-66-2001-050	S5a [GST-tagged]	50 ug	Datasheet
UBI-66-2002-050	S5a [untagged]	50 ug	Datasheet