S5a [untagged]

Ubiquigent

Catalog No: UBI-66-2002-050

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Species Reactivity: human  
Source: E. coli  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  
Concentration: 0.5mg/ml  
Molecular Weight: ~41kDa  
Protein Sequence: Accession number: NP_002801. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
Quality Control Activity: Substrate Ubiquitylation Assay: Incubation of S5a for 1 hour at 37 °C in the presence of His- UBE1, His-UBE2W, His-UBE2N and His-UBE2V1, CHIP, ubiquitin and ATP (Lane 1) was compared alongside three control reactions with either ATP (Lane 2), S5a (Lane 3) or CHIP (Lane 4) excluded from the reaction. Ubiquitin conjugates were identified by Western blotting using an anti-S5a antibody and these were observed only in the presence of both ATP and S5a. A degree of E3 (CHIP)-independent ubiquitylation of S5a is also apparent (compare Lane 4 with Lanes 1 and 2).