S5a [GST-tagged]

Ubiquigent

Catalog No: UBI-66-2001-050

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Species Reactivity: human  
Source: E. coli  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  
Concentration: 0.5mg/ml  
Molecular Weight: ~67.6kDa  
Protein Sequence: Accession number: NP_002801. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
Quality Control Activity: Substrate Ubiquitylation Assay: Incubation of GST-S5a for 1 hour at 37 °C in the presence of His-UBE1, His-UBE2W, His-UBE2N and His-UBE2V1, CHIP, ubiquitin and ATP (Lane 1) was compared alongside three control reactions with either ATP (Lane 2), GST-S5a (Lane 3) or CHIP (Lane 4) excluded from the reaction. Ubiquitin conjugates were identified by Western blotting using an anti- S5a antibody and these were observed only in the presence of both ATP and GST-S5a. A degree of E3 (CHIP)-independent ubiquitylation of GST-S5a is also apparent (compare Lane 4 with Lanes 1 and 2).