Atelocollagen, Permeable Membrane for 6-well, 24-well Culture Plate

Atelocollagen, Permeable Membrane for 6-well, 24-well Culture Plate

 

 Features and Applications

Culture on membrane	Co-culture	Cell sheet transplantation

• Since the collagen membrane is fully permeable to small molecules, cells cultured on this membrane will maintain their polarity and absorb nutrients/excrete metabolic waste products across the membrane.
• Because the membrane is transparent, microscopic observation of the cells is possible during culture.
• Two different cell types can be cultured, one on each side of the membrane, and this membrane is particularly useful for cell-cell interaction studies without direct cell-cell contact.
• It is biodegradable and suitable for cell sheet transplantation 

Types of collagen

Bovine dermis derived atelocollagen

Specifications

	CM-6	CM-24
Plate size	For 6-well plate	For 24-well plate
Volume	2,860mm³	470mm³
Culture area	530mm²	64mm²
Frame material	Polystyrene	Polystyrene

 Products

Product name (click for order info)		Cat no (click for datasheet)	Size
Atelocollagen Permeable Membrane (Well Format)		KOU-CM-24	6 Wells
			24 Wells

Frequently Asked Questions

How can I seed cells on Atelocollagen membrane?
Please visit our distributors’ page to download an instruction manual

Why do I have to neutralize Atelocollagen membrane before use?
The membrane is made from atelocollagen acidic solution and neutralization is necessary prior to cell culture.

How can I harvest cells from Atelocollagen membrane?
Use a cell scraper to harvest cells. We have confirmed that the membrane will not degrade upon treatment with lysis buffers contained in nucleic acid extraction kits.

Is it possible to prepare sections from Atelocollagen membrane?
Similar to tissue samples, Atelocollagen membrane can be fixed and/or embedded in paraffin/OCT compound.

How can I observe cells on Atelocollagen membrane?
Cells can be observed using a phase-contrast microscope. We recommend labelling cells beforehand so that you can easily identify cells seeded on each side of the membrane.

What are the differences between MEN-01 and CM-6/CM-24?
The same membrane is used for all three products but the shapes and sizes of the plastic frames holding the membrane are different. We also have Atelocollagen membrane without a plastic frame.

What are the differences between cell culture inserts and Atelocollagen membrane?
Usually a cell culture insert is made from synthetic resin while Atelocollagen membrane is made from 100% collagen. The membrane does not have definitive pores and cells cannot penetrate to the other side of the membrane, yet the membrane is permeable to small molecules. This is why cells can be harvested separately from each side of the membrane following co-culture. The membrane is biodegradable and suitable for cell transplantation.

Is it possible to obtain custom-made Atelocollagen membrane that is different in size and thickness from the off-the-shelf product?
Please contact us as we may be able to produce a custom-made product upon your request. You can also cut the membrane into smaller pieces with a sharp knife such as a surgical scalpel.

References

Co-culture

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• In vitro differentiation of theca cells from ovarian cells isolated from postmenopausal women.
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Transplantation

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Culture on membrane, Miscellaneous

• Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling.
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• Scaffold-free tissue-engineered arterial grafts derived from human skeletal myoblasts.
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• Enzyme-digested Colla Corii Asini (E'jiao) accelerates wound healing and prevents ultraviolet A-induced collagen synthesis decline and wrinkle formation in three-dimensional skin equivalents.
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• Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
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• "All-in-one" in vitro selection of collagen-binding vascular endothelial growth factor.
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• Spontaneous acquisition of infinite proliferative capacity by a rabbit corneal endothelial cell line with maintenance of phenotypic and physiological characteristics.
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• Fluorous solvent effect on cells cultured on collagen membrane at the interface.
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• Role of hepatocyte growth factor in promoting the growth of human corneal endothelial cells stimulated by L-ascorbic Acid 2-phosphate.
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• Effect of lactoferrin-embedded collagen membrane on osteogenic differentiation of human osteoblast-like cells.
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• Immunogold electron microscopic demonstration of distinct submembranous localization of the activated gammaPKC depending on the stimulation.
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• Effects of multipurpose solutions on corneal epithelial tight junctions.
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  Eye Contact Lens. 2008 Jan;34(1):50-55. PMID: 18180685
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