Atelocollagen, Honeycomb Sponge

Honeycomb sponge and Honeycomb disc 96 are sponge shaped type I atelocollagen scaffolds and have a honeycomb-like porous structure and high pore density. This honeycomb structure enables facile supply of nutrients to cells and easy excretion of waste products.

Application and Features


3D Culture	Cell transplantation	Sustained release carrier

The honeycomb structure facilitates the access of nutrients to the cultured cells and the removal of waste products from the sponge, allowing cells to proliferate within the sponge.
Cells are easily harvested by collagenase treatment.
It is biodegradable and suitable for cell transplantation

Type of collagen

Bovine hide derived atelocollagen

Product name (click for order info)	Cat no (click for datasheet)	Size
Atelocollagen Honeycomb sponge	KOU-CSH-10	100mg
Atelocollagen Honeycomb Disc 96	KOU-CSH-96	25 Pieces

Frequently Asked Questions

How can I seed cells into Honeycomb sponge?
Please view the datasheet for the product

What percentage of cells attach onto a Honeycomb sponge during culture?
Approximately 20-30% of seeded cells attach onto the Honeycomb sponge, although the type of cell and the seeding method may affect attachment.

How can I harvest cells from a Honeycomb sponge?
Add collagenase to final concentration of 0.1% and incubate at 37°C for approximately half an hour.

Is it possible to prepare sections from a Honeycomb sponge?
Similar to tissue samples, Honeycomb sponge can be fixed and/or embedded in paraffin/OCT compound.

How long does Honeycomb sponge remain at the transplantation site?
We estimate that it remains for approximately a month but the duration depends on the transplantation site.

Is it possible to obtain custom-made Honeycomb sponges that are different in shape and pore size from the off-the-shelf product?
Please contact us as we may be able to produce custom-made products upon your request. You can also cut Honeycomb sponge into smaller pieces with a sharp knife such as a surgical scalpel.

References

Nerve, Muscle,Blood vessels

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Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and MCP-1 Synergistically Regenerate Transected Rat Peripheral Nerves by Altering Macrophage Polarity.
Kano, F., Matsubara, K., Ueda, M., Hibi, H. and Yamamoto, A.
Stem Cells. 2017 Mar;35(3):641-653. PMID: 27862629
p27(Kip1) and p21(Cip1)-independent proliferative inhibition of vascular smooth muscle cells cultured in type-I collagen matrix honeycombs.
Uchida M, Suzuki S, Suzuki T, Ishii I.
Microvasc Res. 2016 Jan;103:36-40. PMID: 26522285
A 3D nanofibrous hydrogel and collagen sponge scaffold promotes locomotor functional recovery, spinal repair, and neuronal regeneration after complete transection of the spinal cord in adult rats.
Kaneko A, Matsushita A, Sankai Y.
Biomed Mater. 2015 Jan 13;10(1):015008. PMID: 25585935
Bone marrow stromal cells combined with a honeycomb collagen sponge facilitate neurite elongation in vitro and neural restoration in the hemisected rat spinal cord.
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Bone, Tooth, Cartilage

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Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 2018.
Optimization of Extracellular Matrix Synthesis and Accumulation by Human Articular Chondrocytes in 3-Dimensional Construct with Repetitive Hydrostatic Pressure.
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J Biomed Mater Res A. 2007 Feb;80(2):257-67. PMID: 16941596
Mechanism of bone induction by KUSA/A1 cells using atelocollagen honeycomb scaffold.
Hidetsugu T, Paola RA, Hitoshi N, Mehmet G, Jin LY, Borkosky SS, Liliana M, Noriyuki N.
J Biomed Sci. 2007 Mar;14(2):255-263. PMID: 17061146
Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering.
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Differentiation of mesenchymal stem cells into osteoblasts on honeycomb collagen scaffolds.
George J, Kuboki Y, Miyata T.
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Tissue engineering of articular cartilage with autologous cultured adipose tissue-derived stromal cells using atelocollagen honeycomb-shaped scaffold with a membrane sealing in rabbits.
Masuoka K, Asazuma T, Hattori H, Yoshihara Y, Sato M, Matsumura K, Matsui T, Takase B, Nemoto K, Ishihara M.
J Biomed Mater Res B Appl Biomater. 2006 Oct;79(1):25-34. PMID: 16506181
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Arthritis Rheum. 2006 Jun;54(6):1897-905. PMID:16736525
Efficacy of atelocollagen honeycomb scaffold in bone formation using KUSA/A1 cells.　
Rodriguez AP, Missana L, Nagatsuka H, Gunduz M, Tsujigiwa H, Rivera R, Nagai N.
J Biomed Mater Res A. 2006 Jun 15;77(4):707-717. PMID:16555268
An atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS-scaffold) for the culture of annulus fibrosus cells from an intervertebral disc.
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J Biomed Mater Res A. 2003 Feb 1;64(2):248-56. PMID:12522811
Tissue engineering of the intervertebral disc with cultured annulus fibrosus cells using atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS scaffold).
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Med Biol Eng Comput. 2003 May;41(3):365-71. PMID: 12803304

Miscellaneous

Human iPSC-derived neural crest stem cells can produce EPO and induce erythropoiesis in anemic mice.
Brizi V, Buttò S, Cerullo D, Michele Lavecchia A, Rodrigues-Diez R, Novelli R, Corna D, Benigni A, Remuzzi G, Xinaris C.
Stem Cell Res. 2021 Aug;55:102476. PMID: 34339993
神経堤細胞をハニカムに播種し、マウス皮下に移植。
Obesity accelerates hair thinning by stem cell-centric converging mechanisms. Morinaga H, Mohri Y, Grachtchouk M, Asakawa K, Matsumura H, Oshima M, Takayama N, Kato T, Nishimori Y, Sorimachi Y, Takubo K, Suganami T, Iwama A, Iwakura Y, Dlugosz AA, Nishimura EK.
Nature. 2021 Jul;595(7866):266-271. PMID: 34163066.
Recellularization of decellularized cancellous bone scaffolds using low-temperature cell seeding.
Nakamura N, Saito K, Kimura T, Kishida A.
Tissue Cell. 2020 Oct;66:101385. PMID: 32933708.
Development of a cranium-infection rat model for artificial bone implantation.
Inoue Y, Sakamoto Y, Ochiai H, Yoshimura Y, Okumoto T.
Biomed Res Clin Prac, 2016 Volume 1(1): 18-21.
A three-dimensional collagen-sponge-based culture system coated with simplified recombinant fibronectin improves the function of a hepatocyte cell line.
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J Biomed Mater Res B Appl Biomater. 2010 May;93(2):544-550. PMID: 20186828
In vitro and de novo generation of hair from mosaic spheres formed jointly from ES and mesenchymal cells.
Mariko Yamaki.
Journal of the Japanese Society for Regenerative Medicine 2009 8(2):91-97.
Artificial extracellular matrix of type I collagen can suppress the tumorigenetic potential of mouse embryonic stem cells.
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An experimental study of novel bioartificial materials applied to glycotechnology for tissue engineering.
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Embryonic stem cell-derived embryoid bodies in three-dimensional culture system form hepatocyte-like cells in vitro and in vivo.
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Development of composite cultured oral mucosa utilizing collagen sponge matrix and contracted collagen gel: a preliminary study for clinical applications.
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Tissue Eng. 2001 Aug;7(4):415-27. PMID: 11506731
Arg-gingipain a DNA vaccine induces protective immunity against infection by Porphyromonas gingivalis in a murine model.
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