SELECTION BY Product / 3D Ready Atelocollagen

3D Ready Atelocollagen 

 Stable three-dimensional culture (3D culture) can be performed easily without the need for neutralization or mixing with cell culture medium usually required to form atelocollagen gels. High-purity atelocollagen derived from bovine hide is premixed with DMEM, so it can be used immediately. Simply mix with the cells and warm it up. This product is especially recommended for those beginning the 3D culture without worrying about fibril formation during mixing the solutions or cell sedimentation during gel formation. 

Applications and Features

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 3D Culture  Culture on collagen gel Co-culture 
  • Ready-to-use atelocollagen solution for 3D culture.
  • Premixed with DMEM, low glucose or high glucose.
  • Easy operation: simply mix with the cells and warm to form a gel.
  • Reduces cell sedimentation during gel formation achieves a stable 3D culture.

Type of collagen

Bovine hide derived atelocollagen 

References

3D culture

  • COL8A1 enhances the invasion/metastasis in MDA-MB-231 cells via the induction of IL1B and MMP1 expression. Sagara A, Miura S, Kobinata A, Naganawa R, Yaginuma S, Saito S, Saito R, Kominato H, Yumoto T, Sato F. Biochem Biophys Res Commun. 2023 Jan 29;642:145-153. PMID: 36577251.
  • Iwahashi H, Kawashima Y, Masaki H. Photodermatol Photoimmunol Photomed. 2022 Mar;38(2):150-157. PMID: 34469613.
  • BMC Cancer. 2020 Sep 29;20(1):929. PMID: 32993545

Miscellaneous

  • 12-O-Tetradecanoylphorbol 13-acetate promotes proliferation and epithelial-mesenchymal transition in HHUA cells cultured on collagen type I gel: A feasible model to find new therapies for endometrial diseases.
    Hanaki Y, Miyoshi S, Sugiyama Y, Yanagita RC, Sato M.
    Biosci Biotechnol Biochem. 2022 Sep 23;86(10):1417-1422. PMID: 35973688.
  • Newly established patient-derived organoid model of intracranial meningioma.
    Yamazaki S, Ohka F, Hirano M, Shiraki Y, Motomura K, Tanahashi K, Tsujiuchi T, Motomura A, Aoki K, Shinjo K, Murofushi Y, Kitano Y, Maeda S, Kato A, Shimizu H, Yamaguchi J, Adilijiang A, Wakabayashi T, Saito R, Enomoto A, Kondo Y, Natsume A.
    Neuro Oncol. 2021 Nov 2;23(11):1936-1948. PMID: 34214169.

Ordering Information

Product name (click for order info) Cat no (click for datasheet) Size
3D Ready Atelocollagen, DMEM, Low Glucose KOU-3D-LG01  12 ml
3D Ready Atelocollagen, DMEM, Low Glucose KOU-3D-LG01 5 x 12 ml
3D Ready Atelocollagen, DMEM, High Glucose KOU-3D-HG01 12 ml
3D Ready Atelocollagen, DMEM, High Glucose KOU-3D-HG01 5 x 12 ml