KOKEN AteloCell® 3D Collagen Scaffolds
SELECTION GUIDE BY APPLICATION

In vitro applications
Microspheres
High-density cell culture

Membranes
Cell culture ON membranes
Co-culture ACROSS membranes

Sponges and Discs
3D cell culture IN sponges
3D Osteocyte/Bone cultures
3D Mechanical stress cultures

Solutions and Powders
Coating plates with collagen
Culture ON gels
3D culture IN gels

In vivo applications
Cell transplantation
Sustained-release of bioactive compounds
Collagen induced arthritis model
SELECTION BY PRODUCT NAME

3D Ready Atelocollagen

Atelocollagen / Native Collagen Acidic Solutions

Atelocollagen Neutral Solutions

3D Honeycomb Boosted

Atelocollagen, Honeycomb Sponge

Atelocollagen Sponge, MIGHTY

Atelocollagen Coated B-TCP Scaffold

Atelocollagen Microspheres

Collagen Sponge for 35 mm Culture Dish

FibColl® Atelocollagen Inserts 24

Atelocollagen, Permeable Membrane for 50 mm Culture Dish

Atelocollagen, Permeable Membrane for 6-well, 24-well Culture Plate

Type II Collagen

Atelocollagen Powder, Membrane, Sponge

3D Honeycomb Boosted

The 3D Honeycomb Boosted is a carrier for high-density three-dimensional culture (3D culture) made by processing atelocollagen into a spongy scaffold with honeycomb-like through-holes. With a size of Φ5 mm x height 5 mm, which fits in a 96-well plate, it is possible to cultivate 106 cells on a scale approaching that of a 35 mm dish.

3D Honeycomb Boosted


3D Culture	Cell transplantation

The honeycomb structure facilitates the access of nutrients to the cultured cells and the removal of waste products from the sponge, allowing cells to proliferate within the sponge.
It has high resilience to return to its original shape even after compression.
Cells are easily harvested by collagenase treatment.
It is biodegradable and suitable for cell transplantation.
It is immersed in PBS, eliminating the need for static electricity countermeasures and defoaming.

Type of collagen

Bovine hide derived atelocollagen

Product name (click for order info)		Cat no (click for datasheet)	Size
3D Honeycomb Boosted (High density/volume, small pore)		KOU-3D-HCB	25 Pieces

Frequently Asked Questions

How can I seed cells into 3D Honeycomb Boosted?
Please visit our distributors’ page to download an instruction manual.

What percentage of cells attach onto a 3D Honeycomb Boosted?
Approximately 70-80% of seeded cells attach onto the 3D Honeycomb Boosted, although the type of cell and the seeding method may affect attachment.

How can I harvest cells from a 3D Honeycomb Boosted?
Please visit our distributors’ page to download an instruction manual.

Is it possible to prepare sections from a 3D Honeycomb Boosted?
Similar to tissue samples, 3D Honeycomb Boosted can be fixed and/or embedded in paraffin/OCT compound.

What products are used for cell transplantation?
If the site can be incised, an atelocollagen sponge or a membrane, seeded with cells can be used.

　・3D honeycomb Boosted

　・Atelocollagen, Honeycomb sponge/Atelocollagen, Honeycomb Disc 96

　・Atelocollagen Sponge, MIGHTY

　・Atelocollagen, Permeable Membrane

If the incision is unsuitable, cells can be mixed with a high-concentration atelocollagen solution and administered locally for cell transplantation. The atelocollagen forms a gel at the injection site.

　・Atelocollagen Powder

Is it possible to obtain custom-made 3D Honeycomb Boosted that are different in shape and pore size from the off-the-shelf product?
Please contact us as we may be able to produce custom-made products upon your request. You can also cut 3D Honeycomb Boosted into smaller pieces with a sharp knife such as a surgical scalpel.