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Yeastern’s yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5′ – phosphate and the 3′ – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
Features
- Either sticky- or blunt-end, ligation can be achieved within 5 minutes
- The reaction mixture can be used directly for bacterial transformation
Applications
- Join double-strand DNA with cohesive or blunt termini
- Join oligonucleotide linkers to DNA sequence with the blunt end
- Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids
Quality Control
Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.
| Documents & Links for YB Rapid Ligation Kit | |
| Manual | YB Rapid Ligation Kit Manual |
| Vendor Page | YB Rapid Ligation Kit at Yeastern |
| Documents & Links for YB Rapid Ligation Kit | |
| Manual | YB Rapid Ligation Kit Manual |
| Vendor Page | YB Rapid Ligation Kit |