rAAV2_mOaPAC360

Cosmo Bio

Catalog No.: HPK-BD1180A

Plasmid Characterization

There are three plasmids: AAV cis plasmids, containing the vector genome, AAV 
trans plasmids containing rep and cap genes, and adenovirus helper plasmids encoding essential adenovirus 
functions, which are used for the AAV production. Full plasmid characterization includes determination of 
concentration, determination of A260/280 ratio, detection of endotoxin, and plasmid identity. In the plasmid 
identity assay, the presence of each functional DNA element is determined by sequencing and the correct 
restriction pattern is verified with the plasmid map and the seed DNA preparation. For AAV cis-plasmids, these 
elements include the 5’ and 3’ ITR, transgene, promoter, polyadenylation sequence. For AAV trans plasmids, 
the presence of the correct cap gene is verified by full length sequencing. 

Viral Genome (VG) Copy Number Titration

Real time PCR via Applied Biosystems 7900HT are employed to 
determine the viral genome (VG) copy number of AAV vector lots as a measure of AAV particles with full genome 
content. A number of standards, validation samples (both viral and plasmid) and controls (for background and 
DNA contamination) have been used in this assay. 

Endotoxin Assay

The endotoxin assay is carried out using the Limulus Amebocyte Lysate (LAL) gel-clot 
method. Contaminating endotoxin in plasmid preparations can affect transfection efficiencies as well as alter 
the immunogenic properties of the final product. 
 
All the pre-packaged recombinant adeno associated viruses provided by Hamamatsu Photonics K.K. are for 
research application purposes ONLY!