ECOS SONIC Competent Cells (>=1x10^8 cfu/μg)

Yeastern Biotech

Catalog No.: YSN-HYE007-Sonic-5VL

We have taken it upon ourselves to continue the production of Acella™ cells previously from Edge Biosystems Inc., which we have now renamed as ECOS™ Sonic™. Sonic™ Competent Cells allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the Sonic™ strain is a BL21-derivative that features complete deletions of the endA and recA genes, eliminating degradation and recombination of DNA. For optimal results, recommended for use in combination with Yeastern’s proprietary T&A™ expression vector, which will combine transformation, cloning and protein expression into one single step.

Features

• Fast: Only 4-6hrs culture time required to isolates plasmid DNA
• Stable: Lack of ompT and Ion proteases to stabilize synthesized protein; deletion of endA and recA eliminates DNA degradation and recombination
• High efficiency and protein yield: 1 x 108 cfu/ug
• Simple to use: clone and express with the same strain, no more sub-cloning required!

Applications

• protein expression/ toxic protein expression
• preservation of the PCR product
• preservation of the expression plasmid

Genotype

F– ompT hsdsB (rB–mB–) gal dcm (DE3) ΔendA ΔrecA

Specifications

• Efficiency >1 x 10^8 cfu/μg
• 100 μl/vial (5 or 20 vials)
• Control Plasmid: (pUC19)