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We have taken it upon ourselves to continue the production of Acella™ cells previously from Edge Biosystems Inc., which we have now renamed as ECOS™ Sonic™
Sonic™ Competent Cells allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the Sonic™ strain is a BL21-derivative that features complete deletions of the endA and recA genes, eliminating degradation and recombination of DNA.
For optimal results, recommended for use in combination with Yeastern’s proprietary T&A™ expression vector, which will combine transformation, cloning and protein expression into one single step.
Features
Fast: Only 4-6hrs culture time required to isolates plasmid DNA
Stable: Lack of ompT and Ion proteases to stabilize synthesized protein; deletion of endA and recA eliminates DNA degradation and recombination
High efficiency and protein yield: 1 x 108 cfu/ug
Simple to use: clone and express with the same strain, no more sub-cloning required!
Applications
- protein expression/ toxic protein expression
- preservation of the PCR product
- preservation of the expression plasmid
Genotype
F– ompT hsdsB (rB–mB–) gal dcm (DE3) ΔendA ΔrecA
Specifications
- Efficiency >1 x 10^8 cfu/μg
- 100 μl/vial (5 or 20 vials)
- Control Plasmid: (pUC19)
| Documents & Links for ECOS SONIC Competent Cells (>=1x10^8 cfu/μg) | |
| Manual | ECOS™ SONIC Competent Cells (>=1x10^8 cfu/μg) Manual |
| Vendor Page | ECOS SONIC Competent Cells (>=1x10^8 cfu/μg) at Yeastern |
| Documents & Links for ECOS SONIC Competent Cells (>=1x10^8 cfu/μg) | |
| Manual | ECOS™ SONIC Competent Cells (>=1x10^8 cfu/μg) Manual |
| Vendor Page | ECOS SONIC Competent Cells (>=1x10^8 cfu/μg) |