ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg)

Catalog No:
YSN-FYE709-10VL
$221.00

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JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA and the production of ssDNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.

This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

Features
  • Rapid growing strain
  • Carries F’ episome
Applications
  • Preparation of ssDNA
  • Construction of gene library
  • Subcloning
Genotype

e14(McrArecA1 endA1 gyrA96 thi-1 hsdR17(rK mK+supE44 relA1 D(lacproAB) [F’ traD36 proAB lacIqZDM15]

Specifications

  • Efficiency >5 x 10^7 cfu/μg
  • 100 μl/vial (10 or 80 vials)
  • Control Plasmid: (pUC19)
Documents & Links for ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg)
Manual ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg) Manual
Vendor Page ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg) at Yeastern

Documents & Links for ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg)
Manual ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg) Manual
Vendor Page ECOS 9-5 JM109 Competent Cells (>=5 x10^8 cfu/μg)