ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)

Yeastern Biotech

Catalog No.:
YSN-FYE207-10VL
ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)
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This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.

Features
  • lack of Lon and Omp proteases allows high-level protein expression and accumulation
  • Easy gene induction
Applications
  • IPTG induced gene expression in E. coli
  • Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
  • Subcloning
Genotype

F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Specifications

  • Efficiency >2 x 10^7 cfu/μg
  • 100 μl/vial (10 or 40 vials)
  • Control Plasmid: (pUC19)



Documents & Links for ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)
Manual ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) Manual
Vendor Page ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) at Yeastern Biotech

Documents & Links for ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)
Manual ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) Manual
Vendor Page ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg)
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