Click here to view more products from Yeastern Biotech on the YSN Dashboard Page.
View other Transfections products hereThis strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Features
- lack of Lon and Omp proteases allows high-level protein expression and accumulation
- Easy gene induction
Applications
- IPTG induced gene expression in E. coli
- Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
- Subcloning
Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Specifications
- Efficiency >2 x 10^7 cfu/μg
- 100 μl/vial (10 or 40 vials)
- Control Plasmid: (pUC19)
| Documents & Links for ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) | |
| Manual | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) Manual |
| Vendor Page | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) at Yeastern |
| Documents & Links for ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) | |
| Manual | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) Manual |
| Vendor Page | ECOS 21 BL21(DE3) Competent Cells (>=2x10^7 cfu/μg) |