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DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Features
Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.
Applications
- Cloning and subcloning
- Scale-up application
- Blue/white screening
Genotype
endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–
Specifications
- Efficiency >1 x 108 cfu/μg
- 100 μl/vial (10 or 80 vials)
- Control Plasmid: (pUC19)
| Documents & Links for ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg) | |
| Manual | ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg) Manual |
| Vendor Page | ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg) at Yeastern |
| Documents & Links for ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg) | |
| Manual | ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg) Manual |
| Vendor Page | ECOS 101 DH5α Competent Cells (>=1x10^8 cfu/μg) |