Anti Keratin, Type II Cytoskeletal 8 (Keratin-8) mAb (Clone RL273)

Cosmo Bio

SKU:
CAC-NU-01-KE8
  • Figure 1. Western blot analysis.
Whole cell lysate prepared from DLD-1 human colorectal adenocarcinoma cell line was immunoblotted with RL273 antibody (1:200 dilution). The antibody detected a polypeptide band for keratin 8 at approximately 50 kDa.
  • Figure 2. Immunofluorescence microscopy of rabbit colon and DLD-1 cells.Frozen sections of rabbit colon were stained by RL273 antibody at 1:250 dilution (A). The absorptive epithelial cells and mucosal gland cells were stained by the antibody. Panel B shows the phase contrast image of A. Bar = 100 um. Acetone-fixed DLD-1 cells were stained by RL273 antibody at 1:250 dilution (C), showing the filamentous networks of keratins (green). Cell nuclei were visualized by DAPI (blue). Bar = 25 um.
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Hemidesmosomes are adhesive structures between cells and the extracellular matrix. They play a role in anchoring intermediate fibers to the extracellular basement membrane. Structurally, hemidesmosomes occur in two forms: Type I and Type II. Type I hemidesmosomes develop in stratified epithelia such as the epidermis. Its main components include the intracellular linker proteins Plectin and BPAG1, the adhesion receptor integrin α6β4 and collagen type BP180/XVII. Type II hemidesmosomes occur in blood vessels, Schwann cells, and digestive tract epithelia as a simplified form of Type I hemidesmosomes, consisting only plectin and integrin α6β4. The hemidesmosomal adhesion receptor is normally associated with Laminin 5 in the basement membrane. Furthermore, Laminin 5 (of which Laminin gamma 2 is a subunit) is linked to collagen fibers in the dermis via type VII collagen. Genetic deletion of hemidesmosome-related proteins causes various forms of epidermolysis bullosa, highlighting their importance in promoting adhesion between the epidermis and the basement membrane.

Keratin 8 typically dimerizes with Keratin 18 to form an intermediate filament in simple single-layered epithelial cells. Keratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.Together with Keratin 19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.



Product Specifications
Application IF, WB, IHC(f)
Reactivity Rabbit, Human
Clonality Monoclonal (Clone No.: RL273)
Host Mouse


Documents & Links for Anti Keratin, Type II Cytoskeletal 8 (Keratin-8) mAb (Clone RL273)
Datasheet cac-nu-01-ke8_anti-keratin-8-chain-mab-clone-rl273_datasheet.pdf

Documents & Links for Anti Keratin, Type II Cytoskeletal 8 (Keratin-8) mAb (Clone RL273)
Datasheet cac-nu-01-ke8_anti-keratin-8-chain-mab-clone-rl273_datasheet.pdf