Anti CCR9 mAb (Clone 4G2)

CUSABIO

Catalog No.:
CSB-RA004848A0HU-50
Western Blot<br /> Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue<br /> All lanes: CCR9 antibody at 1μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 43, 41 KDa<br /> Observed band size: 43 KDa<br />
Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating CCR9 in HL-60 whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA004848A0HU (3μg) + HL-60 whole cell lysate (500μg)<br /> Lane 3: HL-60 whole cell lysate (20μg)<br />
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Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.

Product Specifications
Application WB, IF, IP, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 4G2)

Documents & Links for Anti CCR9 mAb (Clone 4G2)
Datasheet Anti CCR9 mAb (Clone 4G2) Datasheet

Documents & Links for Anti CCR9 mAb (Clone 4G2)
Datasheet Anti CCR9 mAb (Clone 4G2) Datasheet
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