Anti ATP5B mAb (Clone 5F10)

CUSABIO

Catalog No.:
CSB-RA216446A0HU-50
Western Blot<br /> Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue<br /> All lanes: ATP5F1B antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 57 kDa<br /> Observed band size: 57 kDa<br />
IHC image of CSB-RA216446A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA216446A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human, Mouse, Rat
Clonality Monoclonal (Clone No.: 5F10)

Documents & Links for Anti ATP5B mAb (Clone 5F10)
Datasheet Anti ATP5B mAb (Clone 5F10) Datasheet

Documents & Links for Anti ATP5B mAb (Clone 5F10)
Datasheet Anti ATP5B mAb (Clone 5F10) Datasheet
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