Anti 6-4 Photoproducts (6-4PPs) mAb (Clone 64M-2)

Cosmo Bio LTD

Catalog No: CAC-NM-DND-002

• 
• 
• 

Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.

Application: ICC, ELISA, WB, IP, IHC, FC 
Clonality: Monoclonal 
Host: Mouse 
Purification: Ammonium Sulfate 
Reactivity: All 

Background

Prolonged exposure to solar UV radiation may result in harmful acute and chronic effects to the skin (including skin cancers), eye, and immune system. These harmful effects appear to be closely related to UV-induced DNA damage. Indeed, UV-induced DNA damage plays significant roles in cell-cycle arrest, activation of DNA repair, cell killing, mutation, and neoplastic transformation. The major types of DNA damage induced by solar UV radiation are cyclobutane pyrimidine dimers (CPDs), (6–4) photoproducts (6-4PPs), and Dewar valence isomers of 6-4PPs (Dewar photoproducts; DewarPPs) formed between adjacent pyrimidine nucleotides on the same DNA strand. Approximately 70-80% of UV-induced DNA damage is CPDs and the remaining is 6-4PPs and Dewar isomers of 6-4PPs. DewarPPs are produced by the photoisomerization of 6-4PPs by UV radiation around 325 nm. In normal human cells these types of DNA lesions are repaired by nucleotide excision repair (NER).

To better study molecular events surrounding UV-induced DNA damage and repair, Mori et al. previously developed and characterized monoclonal antibody (mAb) specific for CPDs and mAb specific for 6-4PPs (1) while Matsunaga et al. developed and characterized mAb specific for DewarPPs (2). Three of these antibodies (CPDs: clone TDM-2; 6-4PPs: clone 64M-2; DewarPPs: clone DEM-1) continue to be cited frequently in the literature, often for use in ELISA.

This High Sensitivity (6-4)photoproducts (6-4PPs) ELISA Kit is the only commercially available ELISA utilizing anti- 6-4PPs clone 64M-2 and has been optimized for high sensitivity detection of 6-4PPs in DNA purified from cultured cells or from skin epidermis. This ELISA detects 6-4PPs from dipyrimidines in all DNA sequence contexts (i.e., TT, TC, CT and CC). Thus, the availability and convenience of this ELISA Kit will contribute to further understanding molecular mechanisms involved in cellular responses to UV radiation and DNA damage with applications across many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetics science.

References

1) Yamamoto, A., et al., DNA Repair, 6, 649-657 (2007).
2) Matsumoto, M., et al., J. Cell Sci., 120, 1104-1112 (2007).
3) Yasuda, G., et al., Mol. Cell. Biol., 27, 6606-6614 (2007).
4) Sugasawa, K., et al., Cell 121, 387-400 (2005).
5) Nishiwaki, Y., et al., J. Invest. Dermatol. 122, 526-532 (2004).
6) Imoto, K., et al., J. Invest. Dermatol. 119, 1177-7782 (2002).
7) Wakasugi, M., et al., J. Biol. Chem., 277, 1637-1640 (2002).
8) Kobayashi, N., et al., Pigment Cell Res. 14, 94-102 (2001).
9) Katsumi, S., et al., J. Invest. Dermatol. 117, 1156-1161 (2001).
10) Otoshi, E., et al., Cancer Res. 60, 1729-1735 (2000).
11) Nakagawa, A., et al., J. Invest. Dermatol. 110, 143-148 (1998).
12) Kobayashi, N., et al., J. Invest. Dermatol. 110, 806-810 (1998).
13) Komatsu, Y., et al., Nucleic Acids Res. 25, 3889-3894 (1997).
14) Nakane, H., et al., Nature 377, 165-168 (1995).
15) Todo, T., et al., Nature 361, 371-374 (1993).
16) Kobayashi, N., et al., J. Invest. Dermatol. 101, 685-689 (1993).
17) Potten, C.S., et al., Int. J. Radiat. Biol. 63, 313-324 (1993).
18) Matsunaga, T., et al., Photochem. Photobiol. 54, 403-410 (1991).
19) Mori, T., et al., Photochem. Photobiol. 54, 225-232 (1991).

More than 200 papers using TDM-2 and 64M-2 antibodies have been published so far.

Citations for Anti 6-4 Photoproducts (6-4PPs) mAb (Clone 64M-2) – 34 Found

Yang et al. 2019. Formaldehyde inhibits UV-induced phosphorylation of histone H2AX. Toxicol In Vitro. 61(104687):.   , Journal

Fouquerel et al. 2019. Measuring UV Photoproduct Repair in Isolated Telomeres and Bulk Genomic DNA. Methods Mol Biol. 1999:295-306.   , Journal

Steurer et al. 2019. Fluorescently-labelled CPD and 6-4PP photolyases: new tools for live-cell DNA damage quantification and laser-assisted repair. Nuc Acid Res. 47(7):3536-49.  Journal

Li et al. 2019. Nucleotide excision repair capacity increases during differentiation of human embryonic carcinoma cells into neurons and muscle cells. J Biol Chem. 294(15):5914-5922.   , Journal

Higa et al. 2016. Stabilization of Ultraviolet (UV)-stimulated Scaffold Protein A by Interaction with Ubiquitin-specific Peptidase 7 Is Essential for Transcription-coupled Nucleotide Excision Repair. J Biol Chem. 291(26):13771-9.   , Journal

Kemp et al. 2016. ATR Kinase Inhibition Protects Non-cycling Cells from the Lethal Effects of DNA Damage and Transcription Stress. J Biol Chem. 291(17):9330-42.   , Journal

Canturk et al. 2016. Nucleotide excision repair by dual incisions in plants. PNAS. 113(17):4706-10.   , Journal

Han et al. 2016. Differential DNA lesion formation and repair in heterochromatin and euchromatin. Carcinogenesis. 37(2):129-38.   , Journal

Choi et al. 2015. An Integrated Approach for Analysis of the DNA Damage Response in Mammalian Cells: NUCLEOTIDE EXCISION REPAIR, DNA DAMAGE CHECKPOINT, AND APOPTOSIS. J Biol Chem. 2990(48):28812-21.   , Journal

Schuch et al. 2015. Molecular and sensory mechanisms to mitigate sunlight-induced DNA damage in treefrog tadpoles. J Exp Biol. 218:3059-67.   , Journal

Hu et al. 2015. Genome-wide analysis of human global and transcription-coupled excision repair of UV damage at single-nucleotide resolution. Genes Dev. 29(9):948-60.   , Journal

Matsumoto et al. 2015. Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein. Nucleic Acids Res. 43(3):1700-13.   , Journal

Peacock et al. 2014. DNA repair inhibition by UVA photoactivated fluoroquinolones and vemurafenib. Nucleic Acids Res. 42(22):13714-22.   , Journal

Kemp et al. 2014. DNA repair synthesis and ligation affect the processing of excised oligonucleotides generated by human nucleotide excision repair. J Biol Chem. 289(38):26574-83.   , Journal

Biswas et al. 2014. E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis. Cancer Res. 74(12):3369-77.   , Journal

Lee et al. 2014. The contribution of mitochondrial thymidylate synthesis in preventing the nuclear genome stress. Nucleic Acids Res. 42(8):4972-84.   , Journal

Choi et al. 2014. Highly specific and sensitive method for measuring nucleotide excision repair kinetics of ultraviolet photoproducts in human cells. Nucleic Acids Res. 42(4):e29.   , Journal

Kuschal et al. 2013. Repair of UV photolesions in xeroderma pigmentosum group C cells induced by translational readthrough of premature termination codons. PNAS. 110(48):19483-8.   , Journal

Hu et al. 2013. Nucleotide excision repair in human cells: fate of the excised oligonucleotide carrying DNA damage in vivo. J Biol Chem. 288(29):20918-26.   , Journal

Jarrett et al. 2012. Metastasis suppressor NM23-H1 promotes repair of UV-induced DNA damage and suppresses UV-induced melanomagenesis. Cancer Res. 72(1):133-43.   , Journal

Gaddameedhi et al. 2011. Control of skin cancer by the circadian rhythm. PNAS. 108(46):18790-5.   , Journal

Burgess et al. 2011. Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export of metazoan PABPs. J Cell Sci. 124:3344-55.  Journal

Renaud et al. 2011. Differential contribution of XPC, RAD23A, RAD23B and CENTRIN 2 to the UV-response in human cells. DNA Repair (Amst). 10(8):835-47.   , Journal

Soares et al. 2011. Trabectedin and its C subunit modified analogue PM01183 attenuate nucleotide excision repair and show activity toward platinum-resistant cells. Mol Cancer Ther. 10(8):1481-9.   , Journal

Han et al. 2011. Caffeine promotes ultraviolet B-induced apoptosis in human keratinocytes without complete DNA repair. J Biol Chem. 286(26):22825-32.   , Journal

Guervilly et al. 2011. USP1 deubiquitinase maintains phosphorylated CHK1 by limiting its DDB1-dependent degradation. Hum Mol Genet. 20(11):2171-81.   , Journal

Kang et al. 2011. Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein. Nucleic Acids Res. 39(8):3176-87.   , Journal

Kraft et al. 2011. Immunological detection of UV induced cyclobutane pyrimidine dimers and (6-4) photoproducts in DNA from reference bacteria and natural aquatic populations. J Microbiol Methods. 84(3):435-41.   , Journal

Saijo et al. 2011. Nucleotide excision repair by mutant xeroderma pigmentosum group A (XPA) proteins with deficiency in interaction with RPA. J Biol Chem. 286(7):5476-83.   , Journal

Overmeer et al. 2011. Replication protein A safeguards genome integrity by controlling NER incision events. J Cell Biol. 192(3):401-15.   , Journal

Ming et al. 2010. Regulation of global genome nucleotide excision repair by SIRT1 through xeroderma pigmentosum C. PNAS. 107(52):22623-8.   , Journal

Overmeer et al. 2010. Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment. Mol Cell Biol. 30(20):4828-39.   , Journal

Duan et al. 2010. UV damage in DNA promotes nucleosome unwrapping. J Biol Chem. 285(34):26295-303.   , Journal

Fan et al. 2010. SIRT1 regulates UV-induced DNA repair through deacetylating XPA. Mol Cell. 39(2):247-58.   , Journal

Choose Options

High Sensitivity 6-4 Photoproducts (6-4PPs) ELISA Kit

Cosmo Bio LTD

MSRP:

Was:

Now: $976.00

Choose Options

High Sensitivity 6-4PP/(6-4) Photoproduct ELISA Kit (TMB)

Cosmo Bio LTD

MSRP:

Was:

Now: $976.00

Choose Options

Anti Dewar Photoproducts (DewarPPs) mAb (Clone DEM-1)

Cosmo Bio LTD

MSRP:

Was:

Now: $461.00

Choose Options

High Sensitivity Cyclobutane Pyrimidine Dimers (CPDs) ELISA Kit Ver. 2

Cosmo Bio LTD

MSRP:

Was:

Now: $976.00