"Linking Ubiquitin Research to Drug Discovery"

Ubiquigent - E3 Ligases

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E3 ligases confer specificity to the ubiquitylation pathway by recognizing target substrates and mediating transfer of ubiquitin or ubiquitin like modifiers (UBLs) from an E2 conjugating enzyme to a substrate. There are estimated to be >700 E3 ligases forming several classes of enzyme; N-end rule ubiquitin E3 ligases, Homology to E6AP C-Terminus (HECT) domain E3s, Really Interesting New Gene (RING) domain E3s, U-Box containing E3s, Inhibitor of Apoptosis (IAP) E3s, and Cullin-RING E3 ligases (CRLs). The classes of E3 ligases are continually expanding. HECT E3 ligases form a thioester intermediate with the C-terminus of activated ubiquitin via a catalytic cysteine residue on the E3. In this case, ubiquitin is transferred from an E2 via the E3 to a substrate protein lysine side chain. RING and U-Box E3 ligases do not possess a catalytic cysteine residue and bring the E2-ubiquitin complex and substrate into close proximity to mediate the transfer of the ubiquitin directly from the E2 to the substrate.
Catalog Number Product Name Size Datasheet
UBI-63-0001-025 CHIP [6His-tagged] 25 ug Datasheet
UBI-63-0002-025 CHIP [GST-tagged] 25 ug Datasheet
UBI-63-0003-020 CHIP [untagged] 100 ug Datasheet
UBI-63-0003-020 CHIP [untagged] 20 ug Datasheet
UBI-63-0004-010 CBL [GST-tagged] 10 ug Datasheet
UBI-63-0004-010 CBL [GST-tagged] 50 ug Datasheet
UBI-63-0006-025 ITCH [GST-tagged] 25 ug Datasheet
UBI-63-0015-025 BIRC2 [GST-tagged] 25 ug Datasheet
UBI-63-0018-025 IPAH9.8 [GST-tagged] 25 ug Datasheet
UBI-63-0021-025 RNF8 [GST-tagged] 25 ug Datasheet
UBI-63-0023-025 RNF11 [6His-tagged] 25 ug Datasheet
UBI-63-0024-025 RNF11 [GST-tagged] 25 ug Datasheet
UBI-63-0027-025 SMURF1 [GST-tagged] 25 ug Datasheet
UBI-63-0029-025 TRIAD1 [6His-tagged] 25 ug Datasheet
UBI-63-0033-025 WWP1 [GST-tagged] 25 ug Datasheet
UBI-63-0034-025 WWP1 [untagged] 25 ug Datasheet
UBI-63-0038-025 NLEL [GST-tagged] 25 ug Datasheet
UBI-63-0040-025 ZNRF1 [GST-tagged] 25 ug Datasheet
UBI-63-0041-025 ZNRF2 [GST-tagged] 25 ug Datasheet
UBI-63-0042-025 HUWE1 [GST-tagged] 25 ug Datasheet
UBI-63-0044-025 IDOL [6His-tagged] 25 ug Datasheet
UBI-63-0046-025 SMURF2 [GST-tagged] 25 ug Datasheet
UBI-63-0047-025 MuRF1 [untagged] 25 ug Datasheet
UBI-63-0048-025 Parkin [untagged] 25 ug Datasheet
UBI-63-1000-025 Cul1/Rbx1 [untagged] 25 ug Datasheet
UBI-63-1001-025 Cul1/Rbx1/Skp1 [untagged] 25 ug Datasheet
UBI-63-1002-025 Cul5/Rnf7 [untagged] 25 ug Datasheet
UBI-63-1003-025 Cul3/Rbx1 [untagged] 25 ug Datasheet
UBI-63-1004-025 Cul2/Rbx1 [untagged] 25 ug Datasheet
UBI-63-2000-025 DCNL1 [6His-tagged] 25 ug Datasheet
UBI-63-2001-025 DCNL1 [GST-tagged] 25 ug Datasheet
UBI-63-2002-025 DCNL2 [GST-tagged] 25 ug Datasheet
UBI-63-2004-025 DCNL2 [6His-tagged] 25 ug Datasheet