Easy measurement of "acidic mucopolysaccharides" produced from cultured cartilage cells

Acid mucopolysaccharide assay kit

The acidic mucopolysaccharide quantification kit can easily quantify the amount of "acidic mucopolysaccharide" produced from cultured cartilage cells using a spectrophotometer.

Intended use

Chondrocytes are known to secrete large amounts of extracellular matrices such as collagen and mucopolysaccharides as they differentiate.
Acidic mucopolysaccharides are sometimes quantified as an indicator of chondrocyte differentiation, and methods using isotopes and methods of quantifying by HPLC are known.

Compared to the above method, this kit is simpler and allows simple quantification of acidic mucopolysaccharides by combining a coloring solution and a buffer solution with a characteristic composition.

Features

  • Easy and simple quantification can be performed using a visible spectrophotometer.

Composition

For 100 samples (5 times)

  • Coloring solution 4 mL 1 bottle
  • 130mL buffer solution
  • Enzyme powder for specimen preparation 5 for 10 mL
  • Standard solution (chondroitin sulfate 100 μg/mL) 2 mL x 1
References
  1. Obayashi, K., Miyagawa-Tomita, S., Matsumoto, H., Koyama, H., Nakanishi, T., Hirose, H.
    Effects of Transforming Growth Factor-beta3 and Matrix Metalloproteinase-3 on The Pathogenesis of Chronic Mitral Valvular Disease in Dogs.
    Am. J. Vet. Res. 72, 194-202 (2011)
  2. Nakamichi, R., Ito, Y., Inui, M., Onizuka, N., Kayama, T., Kataoka, K., Suzuki, H., Mori, M., Inagawa, M., Ichinose, S., Lotz, MK., Sakai, D., Masuda, K., Ozaki, T., Asahara, H.
    Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs.
    Nat. Commun. 7, 12503 (2016), (PMID:27527664)
  3. Tanaka, Y., Shirasawa, B., Takeuchi, Y., Kawamura, D., Nakamura, T., Samura, M., Nishimoto, A., Ueno, K., Morikage, N., Hosoyama, T., Hamano, K..
    Autologous preconditioned mesenchymal stem cell sheets improve left ventricular function in a rabbit old myocardial infarction model.
    Am. J. Transl. Res. 8, 2222-2233 (2016), (PMID:27347329)

 

Catalog Number Product Name Size
PMC-AK03 Acidic Mucopolysaccharide Assay Kit 100 rxns