Anti Plectin (PCN/PLTN) mAb (Clone PN753)

Cosmo Bio

SKU:
CAC-NU-01-PLN
  • Figure 1. Western blot analysis.
Whole cell lysates prepared from DJM-1 cells (lane 1) and HeLa cells (lane 2) were separated by SDS-PAGE (5% gel) and immunoblotted with PN753 or PC742 (1: 200 dilution). Plectin antibodies detected approximately 500 kDa sized bands (arrow). The smaller polypeptide found in lane 2 may be a degraded product or alternatively spliced ​​rod-less isoform of plectin.
  • Figure 2. The locations of epitopes for Plectin antibodies.Clones PN753 and PC742 were obtained by immunizing mice with the NH2- (173-595 aa) or the COOH-terminal (2,930-3,153 aa) regions of human Plectin (4,574 aa). Grey box represents a predicted coiled-coil region (1,300-2,600 aa).
  • Figure 3. Immunofluorescence microscopy of human skin.Human skin sections were stained with PC742 (1:100 dilution) or PN753 (1:100 dilution). Arrows indicate dermal-epidermal junctions. PN753 stains epidermal cells in addition to hemidesmosomes at the dermal-epidermal junction. Section were fixed with -20°C acetone for 10 min. Bar: 50 um.
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Hemidesmosomes are adhesive structures between cells and the extracellular matrix. They play a role in anchoring intermediate fibers to the extracellular basement membrane. Structurally, hemidesmosomes occur in two forms: Type I and Type II. Type I hemidesmosomes develop in stratified epithelia such as the epidermis. Its main components include the intracellular linker proteins Plectin and BPAG1, the adhesion receptor integrin α6β4 and collagen type BP180/XVII. Type II hemidesmosomes occur in blood vessels, Schwann cells, and digestive tract epithelia as a simplified form of Type I hemidesmosomes, consisting only plectin and integrin α6β4. The hemidesmosomal adhesion receptor is normally associated with Laminin 5 in the basement membrane. Furthermore, Laminin 5 (of which Laminin gamma 2 is a subunit) is linked to collagen fibers in the dermis via type VII collagen. Genetic deletion of hemidesmosome-related proteins causes various forms of epidermolysis bullosa, highlighting their importance in promoting adhesion between the epidermis and the basement membrane.

Plectin interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.

 

References:

1) Yamauchi T., et al. J. Dermatol. Sci., 76:25-33 (2014).

2) Hirako Y., et al. Exp. Cell Res., 324:172-182 (2014).

3) Hirako Y., et al. J. Biol. Chem., 273:9711-9717 (1998).



Product Specifications
Application IP, IF, WB
Reactivity Porcine, Rabbit, Rat, Human
Clonality Monoclonal (Clone No.: PN753)
Host Mouse


Documents & Links for Anti Plectin (PCN/PLTN) mAb (Clone PN753)
Datasheet cac-nu-01-pln_anti-plectin-mab-clone-pn753_datasheet.pdf

Documents & Links for Anti Plectin (PCN/PLTN) mAb (Clone PN753)
Datasheet cac-nu-01-pln_anti-plectin-mab-clone-pn753_datasheet.pdf