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To deliver antibodies into living cell. It can easily introduce antibodies into living cell effcientlly by usuing function of artificial cell penetrating peptide, controlling cytotoxicity.
Features
- Suitable for use with 10% serum-containing media.
- Suitable for various antibodies(polyclonal, monoclonal). It is also possible to introduce fluorescently labeled antibodies and enzyme-labeled antibodies.
- Stable at 4 ° C for 6 months. The activity does not decrease even after freezing and thawing (10 times).
- Cell lines with a proven track record (will be added at any time)
- Ready-to-use. Easy and quick procedure.
- Very low cytotoxicity.
Specificity: Endotxin level < 0.05 EU / ul
Antibody transfectionactivity (relative value)* 80 - 150 %
* (BETA) -galactosidase-conjugated goat IgG was transfected to HeLa cells and incubated for 4 hrs. Antibody transfection activity was estimated by (BETA)- galactosidase activity in lysates of the cells. The values represent relative activity (%) to that of Lot N12J15 as standard.
References
1) FEBS Journal (2015) 282: 142-152.
Effective capture of proteins inside living cells by antibodies indirectly linked to a novel cell-penetrating polymer-modified protein A derivative.
Shoko Itakura, Susumu Hama, Hisafumi Ikeda, Naoto Mitsuhashi, Eiji Majima and Kentaro Kogure.
Shoko Itakura, Susumu Hama, Hisafumi Ikeda, Naoto Mitsuhashi, Eiji Majima and Kentaro Kogure.
2) Mol Cell Biochem (2015) 404: 25-30.
Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells.
Kana Kuwahara, Kazuki Harada, Ryohei Yamagoshi, Takenori Yamamoto, Yasuo Shinohara.
Kana Kuwahara, Kazuki Harada, Ryohei Yamagoshi, Takenori Yamamoto, Yasuo Shinohara.
| Documents & Links for Ab-Carrier | |
| Datasheet | ptn-p-101-25_ab-carrier_datasheet.pdf |
| Flyer | Ab-Carrier Flyer |
| Documents & Links for Ab-Carrier | |
| Datasheet | ptn-p-101-25_ab-carrier_datasheet.pdf |
| Flyer | Ab-Carrier Flyer |