UBE2Q1 (NICE-5) [GST-tagged]

Ubiquigent

Catalog No: UBI-62-0049-020

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Species Reactivity: Human  
Source: E. coli expression  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  
Concentration: 1 mg/ml  
Molecular Weight: ~73 kDa  
Protein Sequence: Accession number: NP_060052. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
Quality Control Activity: E2-Ubiquitin Thioester Loading Assay: The activity of GST-UBE2Q1 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the GST-UBE2Q1 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and GST-UBE2Q1 enzymes in the presence of ubiquitin and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/GST-UBE2Q1 thioester bond to the reducing agent DTT was confirmed.   

Background

The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including regulated and targeted proteosomal degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). UBE2Q1 is a member of the E2 conjugating enzyme family. Cloning of human UBE2Q1 was first described by Marenholz et al. (2001). UBE2Q1 shares 50-75% sequence identity to its homologues in, Mus musculus, Drosophila, C. elegans and Xenopus. Murine UBE2Q1 has a conserved sequence for ubiquitin binding shared by all the ubiquitin-conjugating enzymes and its NH2-terminal domain appears critical for the binding and internalization of cell surface galactosyltransferase 1 (GalT1) in embryonic stem cells. UBE2Q1 regulates GalT1-associated laminin-dependent embryonic cell adhesion and the formation of embryoid bodies (Wassler et al., 2008).

References:

Marenholz I, Zirra M, Fischer DF, Backendorf C, Ziegler A, Mischke D (2001) Identification of human epidermal differentiation complex (EDC)-encoded genes by subtractive hybridization of entire YACs to a gridded keratinocyte cDNA library. Genome Res 11, 341-55.

Wassler MJ, Shur BD, Zhou W, Geng YJ (2008) Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells. Stem Cells 26, 2006-18.