SAE1 [GST-tagged] / SAE2 [6His-tagged]

Ubiquigent

Catalog No: UBI-61-0005-010

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Species Reactivity: human  

Source: E.coli  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: SAE1 = 64.8 kDa SAE2 = 73.4 kDa  

Protein Sequence: Accession number: SAE1 = NP_005491.1 and SAE2 = NP_005490.1 For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E1 Thioester SUMO Loading Assay: The activity of GST-SAE1/His-SAE2 was validated by loading SUMO1 onto the active cysteine of GST-SAE1/His-SAE2. Incubation of the GST-SAE1/His-SAE2 enzyme in the presence of SUMO1 and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the SUMO / GST-SAE1/His-SAE2 thioester bond to the reducing agent DTT was confirmed.