Anti Mono and Polyubiquitylated conjugates mAb (clone FK2, Biotin-conjugated)

Ubiquigent

Catalog No: UBI-68-0123-025

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Application: ChIP 
Clonality: Monoclonal 
Conjugation: Biotin 
Host: Mouse 
Reactivity: Human 

Source: BALB/C Mouse implantation ascites  
Formula: 10mM phosphate buffer, 0.15M NaCl PH7.4, 0.1% sodium azide  
Concentration: 1 mg/ml  
Molecular Weight: 150kDa  
Protein Sequence: n/a  
Quality Control Protein Identification: n/a  
Quality Control Activity: Anti-Mono and Polyubiquitylated Conjugates mAb (FK2) HRP-linked Antibody Activity Assay: The activity of the anti-mono and polyubiquitylated conjugates mAb (FK2) HRP-linked antibody was tested for its ability to recognise mono and polyubiquitin conjugates but not free ubiquitin. By Western blotting the specific recognition of mono and polyubiquitin conjugates by the antibody over free ubiquitin was demonstrated (Figure 1). In a priming and extension assay containing, UBE1 [6His-tagged] (Cat# 61-0001), UBE2W [6His-tagged] (Cat# 62-0085), UBE2N [untagged] (Cat# 62-0047), UBE2V1 [untagged] (Cat# 62-0059), Ubiquitin (Cat# 60-0001), CHIP [untagged] (Cat# 63-0003) and ATP. Using the anti mono and polyubiquitylated conjugates mAb (FK2) HRP-linked antibody, detection of polyubiquitin chains extending from mono-ubiquitylated CHIP and free chains generated by UBE2N/UBE2V1 in the presence of CHIP were observed. In the absence of CHIP, only the background detection of free polyubiquitin chains generated by UBE2N/UBE2V1 was observed.