Antibodies from Cosmo Bio to Humanin (HN) and potent analog HNG (S14G) accelerate research in cognitive aging and Alzheimer’s disease
Alzheimer’s disease (AD) is a neurodegenerative disease characterized by impairment of cognitive function, death of neuronal cells, the presence of senile plaques and neurofilament tangles. Humanin was originally identified as a mitochondrial genome-encoded 24-residue, secreted peptide possessing potent neuroprotective activity against neuronal cell death from mutant forms of the three known familial AD genes APP, PS1, and PS2, as well as from treatment with Amyloid-beta peptide 1-43 (1). In animal models Humanin has been shown to powerfully protect from cognitive declines associated with both AD and normal aging (2). Neuroprotective effects of Humanin are thought to be exerted extracellularly through interaction with cell surface receptors, including the Formyl Peptide Receptor 2 and tripartite IL6-receptor-related complex comprising CNTF Receptor-Alpha/WSX-1/gp130 (3, 4). Humanin has also been shown to act intracellularly to suppress apoptosis by binding the pro-apoptotic protein Bax (5). Humanin neuroprotective activity is potentiated by binding Insulin-like growth factor-binding protein-3 (6). Intracellular Humanin levels may be regulated by binding the E3 ubiquitin-protein ligase Trim11 (7).
To our knowledge, there is only one source of an antibody to the Humanin analog S14G (also known as HNG) which in multiple assays of neurodegneration is 1000-fold more biologically potent than Humanin itself. Our collection of anti-Humanin antibodies (including anti-S14G) and anti-Humanin ELISA kit are useful in studies of AD as well as of other diseases (such as Type 2 diabetes) where amyloid and amyloid-like deposits cause pathology. In addition, we carry a broad range of useful downstream assays and reagents for study of neurodegenerative diseases such as AD.
References
1. Hashimoto, Y. et al. A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Aβ. Proc Natl Acad Sci USA 98, 6336–6341 (2001).
2. Yen, K. et al. Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans. Sci Rep 8, 14212 (2018).
3. Ying, G. et al. Humanin, a Newly Identified Neuroprotective Factor, Uses the G Protein-Coupled Formylpeptide Receptor-Like-1 as a Functional Receptor. J Immunol 172, 7078–7085 (2004).
4. Hashimoto, Y., Kurita, M., Aiso, S., Nishimoto, I. & Matsuoka, M. Humanin inhibits neuronal cell death by interacting with a cytokine receptor complex or complexes involving CNTF receptor alpha/WSX-1/gp130. Mol Biol Cell 20, 2864–2873 (2009).
5. Guo, B. et al. Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature 423, 456–461 (2003).
6. Ikonen, M. et al. Interaction between the Alzheimer's survival peptide humanin and insulin-like growth factor-binding protein 3 regulates cell survival and apoptosis. Proc Natl Acad Sci USA 100, 13042–13047 (2003).
7. Niikura, T. et al. A tripartite motif protein TRIM11 binds and destabilizes Humanin, a neuroprotective peptide against Alzheimer's disease-relevant insults. Eur. J. Neurosci. 17, 1150–1158 (2003).
Humanin Abs and ELISA kits
Rabbit anti-human Humanin (for WB)
Rabbit anti-human Humanin (for IHC and ELISA)
Rabbit anti-Rattin (rat ortholog of human Humanin; for IHC and EIA)
Human Humanin ELISA kit
DOWNSTREAM ASSAYS AND REAGENTS
Proliferation/Viability assays
MTT Cell Proliferation Assay Kit A
MTT Cell Proliferation Assay Kit B
Blue Cell Viability Assay Kit
ROS Assays
DHR 123 probe (for Intracellular ROS assay)
DHE probe (for Intracellular ROS Assay)
DCFH-DA probe (for Intracellular ROS assay)
Amyloid Beta Peptides
Amyloid (BETA) peptide 40 (A(BETA)40)
Amyloid (BETA) peptide 42 (A(BETA)42)
Amyloid Beta Antibodies and Staining and ELISA Kits
Anti APP (BETA-amyloid precursor protein) mAb (Clone 278)
Anti BETA-Amyloid mAb (Clone 59)
Anti Amyloid BETA Protein (1-40) pAb
Amyloid Fluorescent Staining Kit
Human amyloid beta A4 protein ELISA Kit
Mouse Amyloid beta A4 protein(APP) ELISA kit
Rat Amyloid beta A4 protein(APP) ELISA kit
Human Amyloid beta A4 precursor protein-binding family A member 2(APBA2) ELISA kit
Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Human amyloid beta peptide 1-40,Aβ1-40 ELISA Kit
Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit
Rat amyloid beta peptide 1-40,Aβ1-40 ELISA Kit
Humanin Binding Protein ELISA Kits
FPR2
Human N-formyl peptide receptor 2(FPR2) ELISA kit
Mouse N-formyl peptide receptor 2(FPR2) ELISA kit
gp130
Human glycoprotein 130,gp130 ELISA KIT
Mouse glucoprotein 130,gp130 ELISA KIT
Bax
Human Apoptosis regulator BAX ELISA Kit
Mouse Bcl-2 associated X protein, Bax ELISA Kit
Rat Apoptosis regulator BAX(BAX) ELISA kit
IGFB3
Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit
Mouse Insulin-like growth factor binding protein 3,IGFBP-3 ELISA KIT
Rat Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit
Insulin Like Growth Factor Binding Protein-3 (IGFBP-3) Mouse, ELISA Kit, pink-ONE
Insulin Like Growth Factor Binding Protein-3 (IGFBP-3) Mouse, ELISA Kit
IAPP
Human islet amyloid polypeptide,IAPP ELISA Kit
Microglia ELISA Kits
Human Transmembrane protein 119(TMEM119) ELISA kit
Human Integrin αM,ITG/CD11b ELISA Kit
Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit
Human cluster of differentiation 45(CD45) ELISA Kit
Mouse cluster of differentiation 45(CD45) ELISA Kit
Human Allograft inflammatory factor 1(AIF1) ELISA kit
Mouse Allograft inflammatory factor 1(AIF1) ELISA kit
Human CX3C-chemokine receptor 1,CX3CR1 ELISA Kit
Human Cluster of Differentiation 68(CD68) ELISA kit
Mouse Macrosialin(CD68) ELISA kit
Human soluble CD40 ELISA kit
Mouse Tumor necrosis factor receptor superfamily member 5(CD40) ELISA kit