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Description
Taq Premix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2,
KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure
DNA solutions bacterial colonies and cDNA products.
Applications
·PCR
·Primer Extension
·Colony PCR
·High-Throughput PCR
Concentration: One unit is defined as the amount of enzyme that can incorporate 10nmols of total
dNTPs into an acid-insoluble material in 30 minutes at 74℃ when activated salmon sperm DNA was
used as template / primer.
Quality Assurance: Greater than 95% purity as determined by SDS-PAGE (CBBstaining). The absence
of endonuclease and exonucleases was confirmed.
PCR product: PCR products have one A added at the 3'-terminus. Thus, the PCR product can be used
directly for cloning into a T-vector. Additionally, it is possible to clone the product in blunt-end vectors
after blunting and phosphorylation of the end.
PCR Test: Good amplification result was obtained in PCR reaction using λDNA as a template (Fig.1).
Premix composition：10 mM Tris-HCl, 50 mM MgCl2, 0.2 mM dNTPs, 5 % Glycerol, 0.08 % NP-40,
0.05 % Tween-20, 25 units/ml Taq DNA Polymerase, pH 8.6 @ 25℃
Storage Temperature: - 20℃
| Documents & Links for Taq PCR Premix, Add only template & primers | |
| Datasheet | Taq PCR Premix, Add only template & primers Datasheet |
| Vendor Page | Taq PCR Premix, Add only template & primers at Cosmo Bio LTD |
| Documents & Links for Taq PCR Premix, Add only template & primers | |
| Datasheet | Taq PCR Premix, Add only template & primers Datasheet |
| Vendor Page | Taq PCR Premix, Add only template & primers |