SARS-CoV-2 Spike Glycoprotein ELISA Kit

Hakarel

Catalog No.: HAK-HELSRBD-1

Anti-SARS-CoV-2 spike protein antibody is immobilized on the plate. When the sample is added, the SARS-CoV-2 spike protein in the sample is trapped. After washing, the trapped protein is reacted with an HRP-labeled anti-SARS-CoV-2 spike protein antibody. After substrate addition, the color development by HRP is read and quantified with a plate reader.

Features

• Measures SARS-CoV-2 spike protein in samples such as serum and plasma.
• No special equipment required; measurement is possible with a standard plate reader.
• Sensitive and specific detection using sandwich ELISA with two antibodies against the RBD in the SARS-CoV-2 S1 domain.

The COVID-19 pandemic, which originated in China in 2019, has spread worldwide. As of the end of July 2021, 197.19 million people have been infected and 4.21 million have died. SARS-CoV-2, the causative virus of COVID-19, is a type of coronavirus with an RNA genome of approximately 30,000 base pairs.

The spike protein, encoded by the S gene, is a membrane protein expressed on the virus surface and consists of two domains: S1 and S2. During infection, the S1 domain binds to angiotensin-converting enzyme 2 (ACE2) on the host cell surface, allowing the virus to enter. ACE2 is believed to be the receptor for SARS-CoV-2. Structural analysis has identified a narrow region in S1, the receptor binding domain (RBD), which binds to ACE2.

This product enables measurement of SARS-CoV-2 spike protein in samples using a sandwich ELISA with two high-affinity neutralizing antibodies specific to the RBD in the S1 domain.

Kit Principle

Anti-SARS-CoV-2 spike protein antibody is immobilized on the plate. When a sample is added, the spike protein is trapped. After washing, an HRP-labeled anti-spike protein antibody is added. Upon substrate addition, HRP catalyzes color development, which is then read and quantified with a plate reader.

Additional Features

• Capable of measuring spike protein in serum and plasma samples.
• No need for specialized equipment—standard plate readers suffice.
• High sensitivity and specificity via sandwich ELISA targeting the RBD of the S1 domain.
• Antibodies used are reactive with the Wuhan strain and alpha, beta, gamma, and delta variants (publication in preparati